Single-cell analysis of diverse immune phenotypes in malignant pleural effusion

Zhong-Yin Huang, Ming-Ming Shao, Jian-Chu Zhang, Feng-Shuang Yi, Juan Du, Qiong Zhou, Feng-Yao Wu, Sha Li, Wei Li, Xian-Zhen Huang, Kan Zhai () and Huan-Zhong Shi ()
Additional contact information
Zhong-Yin Huang: Capital Medical University
Ming-Ming Shao: Capital Medical University
Jian-Chu Zhang: Huazhong University of Science and Technology
Feng-Shuang Yi: Capital Medical University
Juan Du: Capital Medical University
Qiong Zhou: Huazhong University of Science and Technology
Feng-Yao Wu: Nanning Fourth People’s Hospital
Sha Li: Nanning Fourth People’s Hospital
Wei Li: Nanning Fourth People’s Hospital
Xian-Zhen Huang: Nanning Fourth People’s Hospital
Kan Zhai: Capital Medical University
Huan-Zhong Shi: Capital Medical University

Nature Communications, 2021, vol. 12, issue 1, 1-12

Abstract: Abstract The complex interactions among different immune cells have important functions in the development of malignant pleural effusion (MPE). Here we perform single-cell RNA sequencing on 62,382 cells from MPE patients induced by non-small cell lung cancer to describe the composition, lineage, and functional states of infiltrating immune cells in MPE. Immune cells in MPE display a number of transcriptional signatures enriched for regulatory T cells, B cells, macrophages, and dendritic cells compared to corresponding counterparts in blood. Helper T, cytotoxic T, regulatory T, and T follicular helper cells express multiple immune checkpoints or costimulatory molecules. Cell-cell interaction analysis identifies regulatory B cells with more interactions with CD4+ T cells compared to CD8+ T cells. Macrophages are transcriptionally heterogeneous and conform to M2 polarization characteristics. In addition, immune cells in MPE show the general up-regulation of glycolytic pathways associated with the hypoxic microenvironment. These findings show a detailed atlas of immune cells in human MPE and enhance the understanding of potential diagnostic and therapeutic targets in advanced non-small cell lung cancer.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27026-9

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DOI: 10.1038/s41467-021-27026-9

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