Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus

Weronika Witkowska McConnell, Chris Davis, Suleman R. Sabir, Alice Garrett, Amanda Bradley-Stewart, Pawel Jajesniak, Julien Reboud, Gaolian Xu, Zhugen Yang, Rory Gunson, Emma C. Thomson () and Jonathan M. Cooper ()
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Weronika Witkowska McConnell: MRC-University of Glasgow, Centre for Virus Research, University of Glasgow
Chris Davis: MRC-University of Glasgow, Centre for Virus Research, University of Glasgow
Suleman R. Sabir: MRC-University of Glasgow, Centre for Virus Research, University of Glasgow
Alice Garrett: University of Glasgow
Amanda Bradley-Stewart: Glasgow Royal Infirmary
Pawel Jajesniak: University of Glasgow
Julien Reboud: University of Glasgow
Gaolian Xu: University of Glasgow
Zhugen Yang: University of Glasgow
Rory Gunson: Glasgow Royal Infirmary
Emma C. Thomson: MRC-University of Glasgow, Centre for Virus Research, University of Glasgow
Jonathan M. Cooper: University of Glasgow

Nature Communications, 2021, vol. 12, issue 1, 1-8

Abstract: Abstract The early diagnosis of active hepatitis C virus (HCV) infection remains a significant barrier to the treatment of the disease and to preventing the associated significant morbidity and mortality seen, worldwide. Current testing is delayed due to the high cost, long turnaround times and high expertise needed in centralised diagnostic laboratories. Here we demonstrate a user-friendly, low-cost pan-genotypic assay, based upon reverse transcriptase loop mediated isothermal amplification (RT-LAMP). We developed a prototype device for point-of-care use, comprising a LAMP amplification chamber and lateral flow nucleic acid detection strips, giving a visually-read, user-friendly result in

Date: 2021
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DOI: 10.1038/s41467-021-27076-z

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