N-terminal tyrosine of ISCU2 triggers [2Fe-2S] cluster synthesis by ISCU2 dimerization

Sven-A. Freibert, Michal T. Boniecki, Claudia Stümpfig, Vinzent Schulz, Nils Krapoth, Dennis R. Winge, Ulrich Mühlenhoff, Oliver Stehling, Miroslaw Cygler () and Roland Lill ()
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Sven-A. Freibert: Philipps-Universität Marburg
Michal T. Boniecki: University of Saskatchewan
Claudia Stümpfig: Philipps-Universität Marburg
Vinzent Schulz: Philipps-Universität Marburg
Nils Krapoth: Philipps-Universität Marburg
Dennis R. Winge: Philipps-Universität Marburg
Ulrich Mühlenhoff: Philipps-Universität Marburg
Oliver Stehling: Philipps-Universität Marburg
Miroslaw Cygler: University of Saskatchewan
Roland Lill: Philipps-Universität Marburg

Nature Communications, 2021, vol. 12, issue 1, 1-15

Abstract: Abstract Synthesis of iron-sulfur (Fe/S) clusters in living cells requires scaffold proteins for both facile synthesis and subsequent transfer of clusters to target apoproteins. The human mitochondrial ISCU2 scaffold protein is part of the core ISC (iron-sulfur cluster assembly) complex that synthesizes a bridging [2Fe-2S] cluster on dimeric ISCU2. Initial iron and sulfur loading onto monomeric ISCU2 have been elucidated biochemically, yet subsequent [2Fe-2S] cluster formation and dimerization of ISCU2 is mechanistically ill-defined. Our structural, biochemical and cell biological experiments now identify a crucial function of the universally conserved N-terminal Tyr35 of ISCU2 for these late reactions. Mixing two, per se non-functional ISCU2 mutant proteins with oppositely charged Asp35 and Lys35 residues, both bound to different cysteine desulfurase complexes NFS1-ISD11-ACP, restores wild-type ISCU2 maturation demonstrating that ionic forces can replace native Tyr-Tyr interactions during dimerization-induced [2Fe-2S] cluster formation. Our studies define the essential mechanistic role of Tyr35 in the reaction cycle of de novo mitochondrial [2Fe-2S] cluster synthesis.

Date: 2021
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DOI: 10.1038/s41467-021-27122-w

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