A genetically encoded fluorescent biosensor for extracellular l-lactate

Yusuke Nasu, Ciaran Murphy-Royal, Yurong Wen, Jordan N. Haidey, Rosana S. Molina, Abhi Aggarwal, Shuce Zhang, Yuki Kamijo, Marie-Eve Paquet, Kaspar Podgorski, Mikhail Drobizhev, Jaideep S. Bains, M. Joanne Lemieux, Grant R. Gordon and Robert E. Campbell ()
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Yusuke Nasu: The University of Tokyo, Bunkyo-ku
Ciaran Murphy-Royal: Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary
Yurong Wen: University of Alberta
Jordan N. Haidey: Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary
Rosana S. Molina: Montana State University
Abhi Aggarwal: Janelia Research Campus, Howard Hughes Medical Institute
Shuce Zhang: University of Alberta
Yuki Kamijo: The University of Tokyo, Bunkyo-ku
Marie-Eve Paquet: CERVO Brain Research Center, Laval University
Kaspar Podgorski: Janelia Research Campus, Howard Hughes Medical Institute
Mikhail Drobizhev: Montana State University
Jaideep S. Bains: Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary
M. Joanne Lemieux: University of Alberta
Grant R. Gordon: Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary
Robert E. Campbell: The University of Tokyo, Bunkyo-ku

Nature Communications, 2021, vol. 12, issue 1, 1-12

Abstract: Abstract l-Lactate, traditionally considered a metabolic waste product, is increasingly recognized as an important intercellular energy currency in mammals. To enable investigations of the emerging roles of intercellular shuttling of l-lactate, we now report an intensiometric green fluorescent genetically encoded biosensor for extracellular l-lactate. This biosensor, designated eLACCO1.1, enables cellular resolution imaging of extracellular l-lactate in cultured mammalian cells and brain tissue.

Date: 2021
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DOI: 10.1038/s41467-021-27332-2

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