Structural basis of Ty3 retrotransposon integration at RNA Polymerase III-transcribed genes

Guillermo Abascal-Palacios (), Laura Jochem, Carlos Pla-Prats, Fabienne Beuron and Alessandro Vannini ()
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Guillermo Abascal-Palacios: The Institute of Cancer Research
Laura Jochem: The Institute of Cancer Research
Carlos Pla-Prats: Friedrich Miescher Institute for Biomedical Research
Fabienne Beuron: The Institute of Cancer Research
Alessandro Vannini: The Institute of Cancer Research

Nature Communications, 2021, vol. 12, issue 1, 1-11

Abstract: Abstract Retrotransposons are endogenous elements that have the ability to mobilise their DNA between different locations in the host genome. The Ty3 retrotransposon integrates with an exquisite specificity in a narrow window upstream of RNA Polymerase (Pol) III-transcribed genes, representing a paradigm for harmless targeted integration. Here we present the cryo-EM reconstruction at 4.0 Å of an active Ty3 strand transfer complex bound to TFIIIB transcription factor and a tRNA gene. The structure unravels the molecular mechanisms underlying Ty3 targeting specificity at Pol III-transcribed genes and sheds light into the architecture of retrotransposon machinery during integration. Ty3 intasome contacts a region of TBP, a subunit of TFIIIB, which is blocked by NC2 transcription regulator in RNA Pol II-transcribed genes. A newly-identified chromodomain on Ty3 integrase interacts with TFIIIB and the tRNA gene, defining with extreme precision the integration site position.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27338-w

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DOI: 10.1038/s41467-021-27338-w

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