Nanosecond-resolution photothermal dynamic imaging via MHZ digitization and match filtering

Jiaze Yin, Lu Lan, Yi Zhang, Hongli Ni, Yuying Tan, Meng Zhang, Yeran Bai and Ji-Xin Cheng ()
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Jiaze Yin: Boston University
Lu Lan: Boston University
Yi Zhang: Boston University
Hongli Ni: Boston University
Yuying Tan: Boston University
Meng Zhang: Boston University
Yeran Bai: Boston University
Ji-Xin Cheng: Boston University

Nature Communications, 2021, vol. 12, issue 1, 1-11

Abstract: Abstract Photothermal microscopy has enabled highly sensitive label-free imaging of absorbers, from metallic nanoparticles to chemical bonds. Photothermal signals are conventionally detected via modulation of excitation beam and demodulation of probe beam using lock-in amplifier. While convenient, the wealth of thermal dynamics is not revealed. Here, we present a lock-in free, mid-infrared photothermal dynamic imaging (PDI) system by MHz digitization and match filtering at harmonics of modulation frequency. Thermal-dynamic information is acquired at nanosecond resolution within single pulse excitation. Our method not only increases the imaging speed by two orders of magnitude but also obtains four-fold enhancement of signal-to-noise ratio over lock-in counterpart, enabling high-throughput metabolism analysis at single-cell level. Moreover, by harnessing the thermal decay difference between water and biomolecules, water background is effectively separated in mid-infrared PDI of living cells. This ability to nondestructively probe chemically specific photothermal dynamics offers a valuable tool to characterize biological and material specimens.

Date: 2021
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DOI: 10.1038/s41467-021-27362-w

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