Hyphal compartmentalization and sporulation in Streptomyces require the conserved cell division protein SepX

Matthew J. Bush, Kelley A. Gallagher, Govind Chandra, Kim C. Findlay and Susan Schlimpert ()
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Matthew J. Bush: John Innes Centre, Norwich Research Park
Kelley A. Gallagher: John Innes Centre, Norwich Research Park
Govind Chandra: John Innes Centre, Norwich Research Park
Kim C. Findlay: John Innes Centre, Norwich Research Park
Susan Schlimpert: John Innes Centre, Norwich Research Park

Nature Communications, 2022, vol. 13, issue 1, 1-13

Abstract: Abstract Filamentous actinobacteria such as Streptomyces undergo two distinct modes of cell division, leading to partitioning of growing hyphae into multicellular compartments via cross-walls, and to septation and release of unicellular spores. Specific determinants for cross-wall formation and the importance of hyphal compartmentalization for Streptomyces development are largely unknown. Here we show that SepX, an actinobacterial-specific protein, is crucial for both cell division modes in Streptomyces venezuelae. Importantly, we find that sepX-deficient mutants grow without cross-walls and that this substantially impairs the fitness of colonies and the coordinated progression through the developmental life cycle. Protein interaction studies and live-cell imaging suggest that SepX contributes to the stabilization of the divisome, a mechanism that also requires the dynamin-like protein DynB. Thus, our work identifies an important determinant for cell division in Streptomyces that is required for cellular development and sporulation.

Date: 2022
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DOI: 10.1038/s41467-021-27638-1

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