NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis

Tomasz Bieluszewski, Weronika Sura, Wojciech Dziegielewski, Anna Bieluszewska, Catherine Lachance, Michał Kabza, Maja Szymanska-Lejman, Mateusz Abram, Piotr Wlodzimierz, Nancy Winne, Geert Jaeger, Jan Sadowski, Jacques Côté () and Piotr A. Ziolkowski ()
Additional contact information
Tomasz Bieluszewski: Adam Mickiewicz University
Weronika Sura: Adam Mickiewicz University
Wojciech Dziegielewski: Adam Mickiewicz University
Anna Bieluszewska: Adam Mickiewicz University
Catherine Lachance: CHU de Québec-UL Research Center-Oncology Division
Michał Kabza: Adam Mickiewicz University
Maja Szymanska-Lejman: Adam Mickiewicz University
Mateusz Abram: Adam Mickiewicz University
Piotr Wlodzimierz: Adam Mickiewicz University
Nancy Winne: VIB-UGent Center for Plant Systems Biology
Geert Jaeger: VIB-UGent Center for Plant Systems Biology
Jan Sadowski: Adam Mickiewicz University
Jacques Côté: CHU de Québec-UL Research Center-Oncology Division
Piotr A. Ziolkowski: Adam Mickiewicz University

Nature Communications, 2022, vol. 13, issue 1, 1-20

Abstract: Abstract Nucleosomal acetyltransferase of H4 (NuA4) is an essential transcriptional coactivator in eukaryotes, but remains poorly characterized in plants. Here, we describe Arabidopsis homologs of the NuA4 scaffold proteins Enhancer of Polycomb-Like 1 (AtEPL1) and Esa1-Associated Factor 1 (AtEAF1). Loss of AtEAF1 results in inhibition of growth and chloroplast development. These effects are stronger in the Atepl1 mutant and are further enhanced by loss of Golden2-Like (GLK) transcription factors, suggesting that NuA4 activates nuclear plastid genes alongside GLK. We demonstrate that AtEPL1 is necessary for nucleosomal acetylation of histones H4 and H2A.Z by NuA4 in vitro. These chromatin marks are diminished genome-wide in Atepl1, while another active chromatin mark, H3K9 acetylation (H3K9ac), is locally enhanced. Expression of many chloroplast-related genes depends on NuA4, as they are downregulated with loss of H4ac and H2A.Zac. Finally, we demonstrate that NuA4 promotes H2A.Z deposition and by doing so prevents spurious activation of stress response genes.

Date: 2022
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DOI: 10.1038/s41467-021-27882-5

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