Structural basis for safe and efficient energy conversion in a respiratory supercomplex

Wei-Chun Kao (), Claire Ortmann de Percin Northumberland, Tat Cheung Cheng, Julio Ortiz, Alexandre Durand, Ottilie Loeffelholz, Oliver Schilling, Martin L. Biniossek, Bruno P. Klaholz and Carola Hunte ()
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Wei-Chun Kao: University of Freiburg
Claire Ortmann de Percin Northumberland: University of Freiburg
Tat Cheung Cheng: Université de Strasbourg
Julio Ortiz: Université de Strasbourg
Alexandre Durand: Université de Strasbourg
Ottilie Loeffelholz: Université de Strasbourg
Oliver Schilling: University of Freiburg
Martin L. Biniossek: University of Freiburg
Bruno P. Klaholz: Université de Strasbourg
Carola Hunte: University of Freiburg

Nature Communications, 2022, vol. 13, issue 1, 1-12

Abstract: Abstract Proton-translocating respiratory complexes assemble into supercomplexes that are proposed to increase the efficiency of energy conversion and limit the production of harmful reactive oxygen species during aerobic cellular respiration. Cytochrome bc complexes and cytochrome aa3 oxidases are major drivers of the proton motive force that fuels ATP generation via respiration, but how wasteful electron- and proton transfer is controlled to enhance safety and efficiency in the context of supercomplexes is not known. Here, we address this question with the 2.8 Å resolution cryo-EM structure of the cytochrome bcc-aa3 (III2-IV2) supercomplex from the actinobacterium Corynebacterium glutamicum. Menaquinone, substrate mimics, lycopene, an unexpected Qc site, dioxygen, proton transfer routes, and conformational states of key protonable residues are resolved. Our results show how safe and efficient energy conversion is achieved in a respiratory supercomplex through controlled electron and proton transfer. The structure may guide the rational design of drugs against actinobacteria that cause diphtheria and tuberculosis.

Date: 2022
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DOI: 10.1038/s41467-022-28179-x

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