Generation of the organotypic kidney structure by integrating pluripotent stem cell-derived renal stroma

Shunsuke Tanigawa, Etsuko Tanaka, Koichiro Miike, Tomoko Ohmori, Daisuke Inoue, Chen-Leng Cai, Atsuhiro Taguchi, Akio Kobayashi and Ryuichi Nishinakamura ()
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Shunsuke Tanigawa: Kumamoto University
Etsuko Tanaka: Kumamoto University
Koichiro Miike: Kumamoto University
Tomoko Ohmori: Kumamoto University
Daisuke Inoue: Kumamoto University
Chen-Leng Cai: Indiana University School of Medicine
Atsuhiro Taguchi: Kumamoto University
Akio Kobayashi: Kumamoto University
Ryuichi Nishinakamura: Kumamoto University

Nature Communications, 2022, vol. 13, issue 1, 1-15

Abstract: Abstract Organs consist of the parenchyma and stroma, the latter of which coordinates the generation of organotypic structures. Despite recent advances in organoid technology, induction of organ-specific stroma and recapitulation of complex organ configurations from pluripotent stem cells (PSCs) have remained challenging. By elucidating the in vivo molecular features of the renal stromal lineage at a single-cell resolution level, we herein establish an in vitro induction protocol for stromal progenitors (SPs) from mouse PSCs. When the induced SPs are assembled with two differentially induced parenchymal progenitors (nephron progenitors and ureteric buds), the completely PSC-derived organoids reproduce the complex kidney structure, with multiple types of stromal cells distributed along differentiating nephrons and branching ureteric buds. Thus, integration of PSC-derived lineage-specific stroma into parenchymal organoids will pave the way toward recapitulation of the organotypic architecture and functions.

Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28226-7

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DOI: 10.1038/s41467-022-28226-7

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