Fast microwave heating-based one-step synthesis of DNA and RNA modified gold nanoparticles

Mengqi Huang, Erhu Xiong (), Yan Wang, Menglu Hu, Huahua Yue, Tian Tian, Debin Zhu, Hong Liu and Xiaoming Zhou ()
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Mengqi Huang: South China Normal University
Erhu Xiong: South China Normal University
Yan Wang: South China Normal University
Menglu Hu: South China Normal University
Huahua Yue: South China Normal University
Tian Tian: South China Normal University
Debin Zhu: South China Normal University
Hong Liu: South China Normal University
Xiaoming Zhou: South China Normal University

Nature Communications, 2022, vol. 13, issue 1, 1-14

Abstract: Abstract DNA/RNA-gold nanoparticle (DNA/RNA-AuNP) nanoprobes have been widely employed for nanobiotechnology applications. Here, we discover that both thiolated and non-thiolated DNA/RNA can be efficiently attached to AuNPs to achieve high-stable spherical nucleic acid (SNA) within minutes under a domestic microwave (MW)-assisted heating-dry circumstance. Further studies show that for non-thiolated DNA/RNA the conjugation is poly (T/U) tag dependent. Spectroscopy, test strip hybridization, and loading counting experiments indicate that low-affinity poly (T/U) tag mediates the formation of a standing-up conformation, which is distributed in the outer layer of SNA structure. In further application studies, CRISPR/Cas9-sgRNA (136 bp), SARS-CoV-2 RNA fragment (1278 bp), and rolling circle amplification (RCA) DNA products (over 1000 bp) can be successfully attached on AuNPs, which overcomes the routine methods in long-chain nucleic acid-AuNP conjugation, exhibiting great promise in biosensing and nucleic acids delivery applications. Current heating-dry strategy has improved traditional DNA/RNA-AuNP conjugation methods in simplicity, rapidity, cost, and universality.

Date: 2022
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DOI: 10.1038/s41467-022-28627-8

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