Digital plasmonic nanobubble detection for rapid and ultrasensitive virus diagnostics

Yaning Liu, Haihang Ye (), HoangDinh Huynh, Chen Xie, Peiyuan Kang, Jeffrey S. Kahn and Zhenpeng Qin ()
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Yaning Liu: University of Texas at Dallas
Haihang Ye: University of Texas at Dallas
HoangDinh Huynh: University of Texas Southwestern Medical Center
Chen Xie: University of Texas at Dallas
Peiyuan Kang: University of Texas at Dallas
Jeffrey S. Kahn: University of Texas Southwestern Medical Center
Zhenpeng Qin: University of Texas Southwestern Medical Center

Nature Communications, 2022, vol. 13, issue 1, 1-11

Abstract: Abstract Rapid and sensitive diagnostics of infectious diseases is an urgent and unmet need as evidenced by the COVID-19 pandemic. Here, we report a strategy, based on DIgitAl plasMONic nanobubble Detection (DIAMOND), to address this need. Plasmonic nanobubbles are transient vapor bubbles generated by laser heating of plasmonic nanoparticles (NPs) and allow single-NP detection. Using gold NPs as labels and an optofluidic setup, we demonstrate that DIAMOND achieves compartment-free digital counting and works on homogeneous immunoassays without separation and amplification steps. DIAMOND allows specific detection of respiratory syncytial virus spiked in nasal swab samples and achieves a detection limit of ~100 PFU/mL (equivalent to 1 RNA copy/µL), which is competitive with digital isothermal amplification for virus detection. Therefore, DIAMOND has the advantages including one-step and single-NP detection, direct sensing of intact viruses at room temperature, and no complex liquid handling, and is a platform technology for rapid and ultrasensitive diagnostics.

Date: 2022
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DOI: 10.1038/s41467-022-29025-w

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