FrCas9 is a CRISPR/Cas9 system with high editing efficiency and fidelity

Zifeng Cui, Rui Tian, Zhaoyue Huang, Zhuang Jin, Lifang Li, Jiashuo Liu, Zheying Huang, Hongxian Xie, Dan Liu, Haiyan Mo, Rong Zhou, Bin Lang, Bo Meng, Haiyan Weng () and Zheng Hu ()
Additional contact information
Zifeng Cui: Sun Yat-sen University
Rui Tian: Sun Yat-sen University, Guangzhou
Zhaoyue Huang: Sun Yat-sen University
Zhuang Jin: Sun Yat-sen University
Lifang Li: Sun Yat-sen University
Jiashuo Liu: Sun Yat-sen University
Zheying Huang: Sun Yat-sen University
Hongxian Xie: Generulor Company Bio-X Lab
Dan Liu: Generulor Company Bio-X Lab
Haiyan Mo: Generulor Company Bio-X Lab
Rong Zhou: Generulor Company Bio-X Lab
Bin Lang: Macao Polytechnic Institute
Bo Meng: University of Science and Technology of China
Haiyan Weng: University of Science and Technology of China
Zheng Hu: Sun Yat-sen University

Nature Communications, 2022, vol. 13, issue 1, 1-12

Abstract: Abstract Genome editing technologies hold tremendous potential in biomedical research and drug development. Therefore, it is imperative to discover gene editing tools with superior cutting efficiency, good fidelity, and fewer genomic restrictions. Here, we report a CRISPR/Cas9 from Faecalibaculum rodentium, which is characterized by a simple PAM (5′-NNTA-3′) and a guide RNA length of 21–22 bp. We find that FrCas9 could achieve comparable efficiency and specificity to SpCas9. Interestingly, the PAM of FrCas9 presents a palindromic sequence, which greatly expands its targeting scope. Due to the PAM sequence, FrCas9 possesses double editing-windows for base editor and could directly target the TATA-box in eukaryotic promoters for TATA-box related diseases. Together, our results broaden the understanding of CRISPR/Cas-mediated genome engineering and establish FrCas9 as a safe and efficient platform for wide applications in research, biotechnology and therapeutics.

Date: 2022
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DOI: 10.1038/s41467-022-29089-8

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