100 Hz ROCS microscopy correlated with fluorescence reveals cellular dynamics on different spatiotemporal scales

Felix Jünger, Dominic Ruh, Dominik Strobel, Rebecca Michiels, Dominik Huber, Annette Brandel, Josef Madl, Alina Gavrilov, Michael Mihlan, Caterina Cora Daller, Eva A. Rog-Zielinska, Winfried Römer, Tim Lämmermann and Alexander Rohrbach ()
Additional contact information
Felix Jünger: University of Freiburg
Dominic Ruh: University of Freiburg
Dominik Strobel: University of Freiburg
Rebecca Michiels: University of Freiburg
Dominik Huber: University of Freiburg
Annette Brandel: University of Freiburg
Josef Madl: University of Freiburg
Alina Gavrilov: Max Planck Institute of Immunobiology and Epigenetics
Michael Mihlan: Max Planck Institute of Immunobiology and Epigenetics
Caterina Cora Daller: University of Freiburg
Eva A. Rog-Zielinska: University of Freiburg
Winfried Römer: University of Freiburg
Tim Lämmermann: Max Planck Institute of Immunobiology and Epigenetics
Alexander Rohrbach: University of Freiburg

Nature Communications, 2022, vol. 13, issue 1, 1-17

Abstract: Abstract Fluorescence techniques dominate the field of live-cell microscopy, but bleaching and motion blur from too long integration times limit dynamic investigations of small objects. High contrast, label-free life-cell imaging of thousands of acquisitions at 160 nm resolution and 100 Hz is possible by Rotating Coherent Scattering (ROCS) microscopy, where intensity speckle patterns from all azimuthal illumination directions are added up within 10 ms. In combination with fluorescence, we demonstrate the performance of improved Total Internal Reflection (TIR)-ROCS with variable illumination including timescale decomposition and activity mapping at five different examples: millisecond reorganization of macrophage actin cortex structures, fast degranulation and pore opening in mast cells, nanotube dynamics between cardiomyocytes and fibroblasts, thermal noise driven binding behavior of virus-sized particles at cells, and, bacterial lectin dynamics at the cortex of lung cells. Using analysis methods we present here, we decipher how motion blur hides cellular structures and how slow structure motions cover decisive fast motions.

Date: 2022
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-022-29091-0 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29091-0

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-022-29091-0

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().