Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats

Angelina Schindele, Fabienne Gehrke, Carla Schmidt, Sarah Röhrig, Annika Dorn and Holger Puchta ()
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Angelina Schindele: Karlsruhe Institute of Technology
Fabienne Gehrke: Karlsruhe Institute of Technology
Carla Schmidt: Karlsruhe Institute of Technology
Sarah Röhrig: Karlsruhe Institute of Technology
Annika Dorn: Karlsruhe Institute of Technology
Holger Puchta: Karlsruhe Institute of Technology

Nature Communications, 2022, vol. 13, issue 1, 1-10

Abstract: Abstract CRISPR/Cas has been mainly used for mutagenesis through the induction of double strand breaks (DSBs) within unique protein-coding genes. Using the SaCas9 nuclease to induce multiple DSBs in functional repetitive DNA of Arabidopsis thaliana, we can now show that cell death can be induced in a controlled way. This approach, named CRISPR-Kill, can be used as tool for tissue engineering. By simply exchanging the constitutive promoter of SaCas9 with cell type-specific promoters, it is possible to block organogenesis in Arabidopsis. By AP1-specific expression of CRISPR-Kill, we are able to restore the apetala1 phenotype and to specifically eliminate petals. In addition, by expressing CRISPR-Kill in root-specific pericycle cells, we are able to dramatically reduce the number and the length of lateral roots. In the future, the application of CRISPR-Kill may not only help to control development but could also be used to change the biochemical properties of plants.

Date: 2022
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DOI: 10.1038/s41467-022-29130-w

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