Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62

Guotai Yu, Oadi Matny, Nicolas Champouret, Burkhard Steuernagel, Matthew J. Moscou, Inmaculada Hernández-Pinzón, Phon Green, Sadiye Hayta, Mark Smedley, Wendy Harwood, Ngonidzashe Kangara, Yajuan Yue, Catherine Gardener, Mark J. Banfield, Pablo D. Olivera, Cole Welchin, Jamie Simmons, Eitan Millet, Anna Minz-Dub, Moshe Ronen, Raz Avni, Amir Sharon, Mehran Patpour, Annemarie F. Justesen, Murukarthick Jayakodi, Axel Himmelbach, Nils Stein, Shuangye Wu, Jesse Poland, Jennifer Ens, Curtis Pozniak, Miroslava Karafiátová, István Molnár, Jaroslav Doležel, Eric R. Ward, T. Lynne Reuber, Jonathan D. G. Jones, Martin Mascher, Brian J. Steffenson () and Brande B. H. Wulff ()
Additional contact information
Guotai Yu: John Innes Centre, Norwich Research Park
Oadi Matny: University of Minnesota
Nicolas Champouret: University of East Anglia
Burkhard Steuernagel: John Innes Centre, Norwich Research Park
Matthew J. Moscou: University of East Anglia
Inmaculada Hernández-Pinzón: University of East Anglia
Phon Green: University of East Anglia
Sadiye Hayta: John Innes Centre, Norwich Research Park
Mark Smedley: John Innes Centre, Norwich Research Park
Wendy Harwood: John Innes Centre, Norwich Research Park
Ngonidzashe Kangara: John Innes Centre, Norwich Research Park
Yajuan Yue: John Innes Centre, Norwich Research Park
Catherine Gardener: John Innes Centre, Norwich Research Park
Mark J. Banfield: John Innes Centre, Norwich Research Park
Pablo D. Olivera: University of Minnesota
Cole Welchin: University of Minnesota
Jamie Simmons: University of Minnesota
Eitan Millet: Tel Aviv University
Anna Minz-Dub: Tel Aviv University
Moshe Ronen: Tel Aviv University
Raz Avni: Tel Aviv University
Amir Sharon: Tel Aviv University
Mehran Patpour: Aarhus University
Annemarie F. Justesen: Aarhus University
Murukarthick Jayakodi: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
Axel Himmelbach: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
Nils Stein: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
Shuangye Wu: Kansas State University
Jesse Poland: Kansas State University
Jennifer Ens: University of Saskatchewan
Curtis Pozniak: University of Saskatchewan
Miroslava Karafiátová: Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural Research
István Molnár: Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural Research
Jaroslav Doležel: Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural Research
Eric R. Ward: University of East Anglia
T. Lynne Reuber: 2Blades Foundation
Jonathan D. G. Jones: University of East Anglia
Martin Mascher: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
Brian J. Steffenson: University of Minnesota
Brande B. H. Wulff: John Innes Centre, Norwich Research Park

Nature Communications, 2022, vol. 13, issue 1, 1-13

Abstract: Abstract The wild relatives and progenitors of wheat have been widely used as sources of disease resistance (R) genes. Molecular identification and characterization of these R genes facilitates their manipulation and tracking in breeding programmes. Here, we develop a reference-quality genome assembly of the wild diploid wheat relative Aegilops sharonensis and use positional mapping, mutagenesis, RNA-Seq and transgenesis to identify the stem rust resistance gene Sr62, which has also been transferred to common wheat. This gene encodes a tandem kinase, homologues of which exist across multiple taxa in the plant kingdom. Stable Sr62 transgenic wheat lines show high levels of resistance against diverse isolates of the stem rust pathogen, highlighting the utility of Sr62 for deployment as part of a polygenic stack to maximize the durability of stem rust resistance.

Date: 2022
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Citations: View citations in EconPapers (7)

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DOI: 10.1038/s41467-022-29132-8

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