Deep-learning two-photon fiberscopy for video-rate brain imaging in freely-behaving mice
Honghua Guan,
Dawei Li,
Hyeon-cheol Park,
Ang Li,
Yuanlei Yue,
Yung-Tian A. Gau,
Ming-Jun Li,
Dwight E. Bergles,
Hui Lu and
Xingde Li ()
Additional contact information
Honghua Guan: Johns Hopkins University
Dawei Li: Johns Hopkins University School of Medicine
Hyeon-cheol Park: Johns Hopkins University School of Medicine
Ang Li: Johns Hopkins University School of Medicine
Yuanlei Yue: School of Medicine and Health Sciences, George Washington University
Yung-Tian A. Gau: Johns Hopkins University School of Medicine
Ming-Jun Li: Science and Technology Division, Corning Incorporated
Dwight E. Bergles: Johns Hopkins University School of Medicine
Hui Lu: School of Medicine and Health Sciences, George Washington University
Xingde Li: Johns Hopkins University
Nature Communications, 2022, vol. 13, issue 1, 1-9
Abstract:
Abstract Scanning two-photon (2P) fiberscopes (also termed endomicroscopes) have the potential to transform our understanding of how discrete neural activity patterns result in distinct behaviors, as they are capable of high resolution, sub cellular imaging yet small and light enough to allow free movement of mice. However, their acquisition speed is currently suboptimal, due to opto-mechanical size and weight constraints. Here we demonstrate significant advances in 2P fiberscopy that allow high resolution imaging at high speeds (26 fps) in freely-behaving mice. A high-speed scanner and a down-sampling scheme are developed to boost imaging speed, and a deep learning (DL) algorithm is introduced to recover image quality. For the DL algorithm, a two-stage learning transfer strategy is established to generate proper training datasets for enhancing the quality of in vivo images. Implementation enables video-rate imaging at ~26 fps, representing 10-fold improvement in imaging speed over the previous 2P fiberscopy technology while maintaining a high signal-to-noise ratio and imaging resolution. This DL-assisted 2P fiberscope is capable of imaging the arousal-induced activity changes in populations of layer2/3 pyramidal neurons in the primary motor cortex of freely-behaving mice, providing opportunities to define the neural basis of behavior.
Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29236-1
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DOI: 10.1038/s41467-022-29236-1
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