The importance of biofilm formation for cultivation of a Micrarchaeon and its interactions with its Thermoplasmatales host

Susanne Krause, Sabrina Gfrerer, Andriko Kügelgen, Carsten Reuse, Nina Dombrowski, Laura Villanueva, Boyke Bunk, Cathrin Spröer, Thomas R. Neu, Ute Kuhlicke, Kerstin Schmidt-Hohagen, Karsten Hiller, Tanmay A. M. Bharat, Reinhard Rachel, Anja Spang and Johannes Gescher ()
Additional contact information
Susanne Krause: Institute of Technology (KIT)
Sabrina Gfrerer: Institute of Technology (KIT)
Andriko Kügelgen: University of Oxford
Carsten Reuse: Technische Universität Braunschweig
Nina Dombrowski: NIOZ, Royal Netherlands Institute for Sea Research
Laura Villanueva: NIOZ, Royal Netherlands Institute for Sea Research
Boyke Bunk: Leibniz Institute DSMZ
Cathrin Spröer: Leibniz Institute DSMZ
Thomas R. Neu: Research UFZ
Ute Kuhlicke: Research UFZ
Kerstin Schmidt-Hohagen: Technische Universität Braunschweig
Karsten Hiller: Technische Universität Braunschweig
Tanmay A. M. Bharat: University of Oxford
Reinhard Rachel: University of Regensburg
Anja Spang: NIOZ, Royal Netherlands Institute for Sea Research
Johannes Gescher: Institute of Technology (KIT)

Nature Communications, 2022, vol. 13, issue 1, 1-15

Abstract: Abstract Micrarchaeota is a distinctive lineage assigned to the DPANN archaea, which includes poorly characterised microorganisms with reduced genomes that likely depend on interactions with hosts for growth and survival. Here, we report the enrichment of a stable co-culture of a member of the Micrarchaeota (Ca. Micrarchaeum harzensis) together with its Thermoplasmatales host (Ca. Scheffleriplasma hospitalis), as well as the isolation of the latter. We show that symbiont-host interactions depend on biofilm formation as evidenced by growth experiments, comparative transcriptomic analyses and electron microscopy. In addition, genomic, metabolomic, extracellular polymeric substances and lipid content analyses indicate that the Micrarchaeon symbiont relies on the acquisition of metabolites from its host. Our study of the cell biology and physiology of a Micrarchaeon and its host adds to our limited knowledge of archaeal symbioses.

Date: 2022
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (3)

Downloads: (external link)
https://www.nature.com/articles/s41467-022-29263-y Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29263-y

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-022-29263-y

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().