Tissue extracellular matrix hydrogels as alternatives to Matrigel for culturing gastrointestinal organoids

Suran Kim, Sungjin Min, Yi Sun Choi, Sung-Hyun Jo, Jae Hun Jung, Kyusun Han, Jin Kim, Soohwan An, Yong Woo Ji, Yun-Gon Kim and Seung-Woo Cho ()
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Suran Kim: Yonsei University
Sungjin Min: Yonsei University
Yi Sun Choi: Yonsei University
Sung-Hyun Jo: Soongsil University
Jae Hun Jung: Yonsei University College of Medicine
Kyusun Han: Yonsei University College of Medicine
Jin Kim: Yonsei University
Soohwan An: Yonsei University
Yong Woo Ji: Yonsei University College of Medicine
Yun-Gon Kim: Soongsil University
Seung-Woo Cho: Yonsei University

Nature Communications, 2022, vol. 13, issue 1, 1-21

Abstract: Abstract Matrigel, a mouse tumor extracellular matrix protein mixture, is an indispensable component of most organoid tissue culture. However, it has limited the utility of organoids for drug development and regenerative medicine due to its tumor-derived origin, batch-to-batch variation, high cost, and safety issues. Here, we demonstrate that gastrointestinal tissue-derived extracellular matrix hydrogels are suitable substitutes for Matrigel in gastrointestinal organoid culture. We found that the development and function of gastric or intestinal organoids grown in tissue extracellular matrix hydrogels are comparable or often superior to those in Matrigel. In addition, gastrointestinal extracellular matrix hydrogels enabled long-term subculture and transplantation of organoids by providing gastrointestinal tissue-mimetic microenvironments. Tissue-specific and age-related extracellular matrix profiles that affect organoid development were also elucidated through proteomic analysis. Together, our results suggest that extracellular matrix hydrogels derived from decellularized gastrointestinal tissues are effective alternatives to the current gold standard, Matrigel, and produce organoids suitable for gastrointestinal disease modeling, drug development, and tissue regeneration.

Date: 2022
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DOI: 10.1038/s41467-022-29279-4

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