Exploring a blue-light-sensing transcription factor to double the peak productivity of oil in Nannochloropsis oceanica

Peng Zhang, Yi Xin, Yuehui He, Xianfeng Tang, Chen Shen, Qintao Wang, Nana Lv, Yun Li, Qiang Hu and Jian Xu ()
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Peng Zhang: Chinese Academy of Sciences
Yi Xin: Chinese Academy of Sciences
Yuehui He: Chinese Academy of Sciences
Xianfeng Tang: University of Chinese Academy of Sciences
Chen Shen: Chinese Academy of Sciences
Qintao Wang: Chinese Academy of Sciences
Nana Lv: Chinese Academy of Sciences
Yun Li: Chinese Academy of Sciences
Qiang Hu: Qingdao National Laboratory for Marine Science and Technology
Jian Xu: Chinese Academy of Sciences

Nature Communications, 2022, vol. 13, issue 1, 1-14

Abstract: Abstract Oleaginous microalgae can produce triacylglycerol (TAG) under stress, yet the underlying mechanism remains largely unknown. Here, we show that, in Nannochloropsis oceanica, a bZIP-family regulator NobZIP77 represses the transcription of a type-2 diacylgycerol acyltransferase encoding gene NoDGAT2B under nitrogen-repletion (N+), while nitrogen-depletion (N−) relieves such inhibition and activates NoDGAT2B expression and synthesis of TAG preferably from C16:1. Intriguingly, NobZIP77 is a sensor of blue light (BL), which reduces binding of NobZIP77 to the NoDGAT2B-promoter, unleashes NoDGAT2B and elevates TAG under N−. Under N+ and white light, NobZIP77 knockout fully preserves cell growth rate and nearly triples TAG productivity. Moreover, exposing the NobZIP77-knockout line to BL under N− can double the peak productivity of TAG. These results underscore the potential of coupling light quality to oil synthesis in feedstock or bioprocess development.

Date: 2022
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DOI: 10.1038/s41467-022-29337-x

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