ComFC mediates transport and handling of single-stranded DNA during natural transformation

Prashant P. Damke, Louisa Celma, Sumedha M. Kondekar, Anne Marie Di Guilmi, Stéphanie Marsin, Jordane Dépagne, Xavier Veaute, Pierre Legrand, Hélène Walbott, Julien Vercruyssen, Raphaël Guérois, Sophie Quevillon-Cheruel () and J. Pablo Radicella ()
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Prashant P. Damke: Université Paris-Saclay, CEA, Stabilité Génétique Cellules Souches et Radiations, Institut de Biologie François Jacob
Louisa Celma: Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC)
Sumedha M. Kondekar: Université Paris-Saclay, CEA, Stabilité Génétique Cellules Souches et Radiations, Institut de Biologie François Jacob
Anne Marie Di Guilmi: Université Paris-Saclay, CEA, Stabilité Génétique Cellules Souches et Radiations, Institut de Biologie François Jacob
Stéphanie Marsin: Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC)
Jordane Dépagne: Université Paris-Saclay, CEA, INSERM, Stabilité Génétique Cellules Souches et Radiations, Institut de Biologie François Jacob
Xavier Veaute: Université Paris-Saclay, CEA, INSERM, Stabilité Génétique Cellules Souches et Radiations, Institut de Biologie François Jacob
Pierre Legrand: L’Orme des Merisiers
Hélène Walbott: Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC)
Julien Vercruyssen: Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC)
Raphaël Guérois: Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC)
Sophie Quevillon-Cheruel: Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC)
J. Pablo Radicella: Université Paris-Saclay, CEA, Stabilité Génétique Cellules Souches et Radiations, Institut de Biologie François Jacob

Nature Communications, 2022, vol. 13, issue 1, 1-13

Abstract: Abstract The ComFC protein is essential for natural transformation, a process that plays a major role in the spread of antibiotic resistance genes and virulence factors across bacteria. However, its role remains largely unknown. Here, we show that Helicobacter pylori ComFC is involved in DNA transport through the cell membrane, and is required for the handling of the single-stranded DNA once it is delivered into the cytoplasm. The crystal structure of ComFC includes a zinc-finger motif and a putative phosphoribosyl transferase domain, both necessary for the protein’s in vivo activity. Furthermore, we show that ComFC is a membrane-associated protein with affinity for single-stranded DNA. Our results suggest that ComFC provides the link between the transport of the transforming DNA into the cytoplasm and its handling by the recombination machinery.

Date: 2022
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DOI: 10.1038/s41467-022-29494-z

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