A synergistic strategy to develop photostable and bright dyes with long Stokes shift for nanoscopy

Gangwei Jiang, Tian-Bing Ren, Elisa D’Este, Mengyi Xiong, Bin Xiong, Kai Johnsson, Xiao-Bing Zhang, Lu Wang () and Lin Yuan ()
Additional contact information
Gangwei Jiang: College of Chemistry and Chemical Engineering, Hunan University
Tian-Bing Ren: College of Chemistry and Chemical Engineering, Hunan University
Elisa D’Este: Max Planck Institute for Medical Research
Mengyi Xiong: College of Chemistry and Chemical Engineering, Hunan University
Bin Xiong: College of Chemistry and Chemical Engineering, Hunan University
Kai Johnsson: Max Planck Institute for Medical Research
Xiao-Bing Zhang: College of Chemistry and Chemical Engineering, Hunan University
Lu Wang: Max Planck Institute for Medical Research
Lin Yuan: College of Chemistry and Chemical Engineering, Hunan University

Nature Communications, 2022, vol. 13, issue 1, 1-10

Abstract: Abstract The quality and application of super-resolution fluorescence imaging greatly lie in the dyes’ properties, including photostability, brightness, and Stokes shift. Here we report a synergistic strategy to simultaneously improve such properties of regular fluorophores. Introduction of quinoxaline motif with fine-tuned electron density to conventional rhodamines generates new dyes with vibration structure and inhibited twisted-intramolecular-charge-transfer (TICT) formation synchronously, thus increasing the brightness and photostability while enlarging Stokes shift. The new fluorophore YL578 exhibits around twofold greater brightness and Stokes shift than its parental fluorophore, Rhodamine B. Importantly, in Stimulated Emission Depletion (STED) microscopy, YL578 derived probe possesses a superior photostability and thus renders threefold more frames than carbopyronine based probes (CPY-Halo and 580CP-Halo), known as photostable fluorophores for STED imaging. Furthermore, the strategy is well generalized to offer a new class of bright and photostable fluorescent probes with long Stokes shift (up to 136 nm) for bioimaging and biosensing.

Date: 2022
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-022-29547-3 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29547-3

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-022-29547-3

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().