Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae

Hui Liu, Pei Zhou, Mengya Qi, Liang Guo, Cong Gao, Guipeng Hu, Wei Song, Jing Wu, Xiulai Chen, Jian Chen, Wei Chen and Liming Liu ()
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Hui Liu: Jiangnan University
Pei Zhou: Jiangnan University
Mengya Qi: Jiangnan University
Liang Guo: Jiangnan University
Cong Gao: Jiangnan University
Guipeng Hu: Jiangnan University
Wei Song: Jiangnan University
Jing Wu: Jiangnan University
Xiulai Chen: Jiangnan University
Jian Chen: Jiangnan University
Wei Chen: Jiangnan University
Liming Liu: Jiangnan University

Nature Communications, 2022, vol. 13, issue 1, 1-14

Abstract: Abstract Saccharomyces cerevisiae is widely employed as a cell factory for the production of biofuels. However, product toxicity has hindered improvements in biofuel production. Here, we engineer the actin cytoskeleton in S. cerevisiae to increase both the cell growth and production of n-butanol and medium-chain fatty acids. Actin cable tortuosity is regulated using an n-butanol responsive promoter-based autonomous bidirectional signal conditioner in S. cerevisiae. The budding index is increased by 14.0%, resulting in the highest n-butanol titer of 1674.3 mg L−1. Moreover, actin patch density is fine-tuned using a medium-chain fatty acid responsive promoter-based autonomous bidirectional signal conditioner. The intracellular pH is stabilized at 6.4, yielding the highest medium-chain fatty acids titer of 692.3 mg L−1 in yeast extract peptone dextrose medium. Engineering the actin cytoskeleton in S. cerevisiae can efficiently alleviate biofuels toxicity and enhance biofuels production.

Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29560-6

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DOI: 10.1038/s41467-022-29560-6

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