Microridge-like structures anchor motile cilia

Takayuki Yasunaga, Johannes Wiegel, Max D. Bergen, Martin Helmstädter, Daniel Epting, Andrea Paolini, Özgün Çiçek, Gerald Radziwill, Christina Engel, Thomas Brox, Olaf Ronneberger, Peter Walentek, Maximilian H. Ulbrich and Gerd Walz ()
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Takayuki Yasunaga: University of Freiburg
Johannes Wiegel: University of Freiburg
Max D. Bergen: University of Freiburg
Martin Helmstädter: University of Freiburg
Daniel Epting: University of Freiburg
Andrea Paolini: University of Freiburg
Özgün Çiçek: University of Freiburg
Gerald Radziwill: BIOSS Centre for Biological Signalling Studies, University of Freiburg
Christina Engel: University of Freiburg
Thomas Brox: University of Freiburg
Olaf Ronneberger: University of Freiburg
Peter Walentek: University of Freiburg
Maximilian H. Ulbrich: University of Freiburg
Gerd Walz: University of Freiburg

Nature Communications, 2022, vol. 13, issue 1, 1-14

Abstract: Abstract Several tissues contain cells with multiple motile cilia that generate a fluid or particle flow to support development and organ functions; defective motility causes human disease. Developmental cues orient motile cilia, but how cilia are locked into their final position to maintain a directional flow is not understood. Here we find that the actin cytoskeleton is highly dynamic during early development of multiciliated cells (MCCs). While apical actin bundles become increasingly more static, subapical actin filaments are nucleated from the distal tip of ciliary rootlets. Anchorage of these subapical actin filaments requires the presence of microridge-like structures formed during MCC development, and the activity of Nonmuscle Myosin II. Optogenetic manipulation of Ezrin, a core component of the microridge actin-anchoring complex, or inhibition of Myosin Light Chain Kinase interfere with rootlet anchorage and orientation. These observations identify microridge-like structures as an essential component of basal body rootlet anchoring in MCCs.

Date: 2022
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DOI: 10.1038/s41467-022-29741-3

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