Snf7 spirals sense and alter membrane curvature

Nebojsa Jukic, Alma P. Perrino, Frédéric Humbert, Aurélien Roux and Simon Scheuring ()
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Nebojsa Jukic: Physiology, Biophysics and Systems Biology Graduate Program, Weill Cornell Medicine
Alma P. Perrino: Weill Cornell Medicine
Frédéric Humbert: University of Geneva
Aurélien Roux: University of Geneva
Simon Scheuring: Weill Cornell Medicine

Nature Communications, 2022, vol. 13, issue 1, 1-17

Abstract: Abstract Endosomal Sorting Complex Required for Transport III (ESCRT-III) is a conserved protein system involved in many cellular processes resulting in membrane deformation and scission, topologically away from the cytoplasm. However, little is known about the transition of the planar membrane-associated protein assembly into a 3D structure. High-speed atomic force microscopy (HS-AFM) provided insights into assembly, structural dynamics and turnover of Snf7, the major ESCRT-III component, on planar supported lipid bilayers. Here, we develop HS-AFM experiments that remove the constraints of membrane planarity, crowdedness, and support rigidity. On non-planar membranes, Snf7 monomers are curvature insensitive, but Snf7-spirals selectively adapt their conformation to membrane geometry. In a non-crowded system, Snf7-spirals reach a critical radius, and remodel to minimize internal stress. On non-rigid supports, Snf7-spirals compact and buckle, deforming the underlying bilayer. These experiments provide direct evidence that Snf7 is sufficient to mediate topological transitions, in agreement with the loaded spiral spring model.

Date: 2022
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DOI: 10.1038/s41467-022-29850-z

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