Periodic formation of epithelial somites from human pluripotent stem cells

Marina Sanaki-Matsumiya (), Mitsuhiro Matsuda, Nicola Gritti, Fumio Nakaki, James Sharpe, Vikas Trivedi and Miki Ebisuya ()
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Marina Sanaki-Matsumiya: European Molecular Biology Laboratory (EMBL) Barcelona
Mitsuhiro Matsuda: European Molecular Biology Laboratory (EMBL) Barcelona
Nicola Gritti: European Molecular Biology Laboratory (EMBL) Barcelona
Fumio Nakaki: European Molecular Biology Laboratory (EMBL) Barcelona
James Sharpe: European Molecular Biology Laboratory (EMBL) Barcelona
Vikas Trivedi: European Molecular Biology Laboratory (EMBL) Barcelona
Miki Ebisuya: European Molecular Biology Laboratory (EMBL) Barcelona

Nature Communications, 2022, vol. 13, issue 1, 1-14

Abstract: Abstract During embryonic development, epithelial cell blocks called somites are periodically formed according to the segmentation clock, becoming the foundation for the segmental pattern of the vertebral column. The process of somitogenesis has recently been recapitulated with murine and human pluripotent stem cells. However, an in vitro model for human somitogenesis coupled with the segmentation clock and epithelialization is still missing. Here, we report the generation of human somitoids, organoids that periodically form pairs of epithelial somite-like structures. Somitoids display clear oscillations of the segmentation clock that coincide with the segmentation of the presomitic mesoderm. The resulting somites show anterior-posterior and apical-basal polarities. Matrigel is essential for epithelialization but dispensable for the differentiation into somite cells. The size of somites is rather constant, irrespective of the initial cell number. The amount of WNT signaling instructs the proportion of mesodermal lineages in somitoids. Somitoids provide a novel platform to study human somitogenesis.

Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29967-1

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DOI: 10.1038/s41467-022-29967-1

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