Cooperative regulation of PBI1 and MAPKs controls WRKY45 transcription factor in rice immunity

Kota Ichimaru, Koji Yamaguchi, Kenichi Harada, Yusaku Nishio, Momoka Hori, Kazuya Ishikawa, Haruhiko Inoue, Shusuke Shigeta, Kento Inoue, Keita Shimada, Satomi Yoshimura, Takumi Takeda, Eiki Yamashita, Toshimichi Fujiwara, Atsushi Nakagawa, Chojiro Kojima () and Tsutomu Kawasaki ()
Additional contact information
Kota Ichimaru: Kindai University, Nakamachi
Koji Yamaguchi: Kindai University, Nakamachi
Kenichi Harada: Osaka University
Yusaku Nishio: Kindai University, Nakamachi
Momoka Hori: Kindai University, Nakamachi
Kazuya Ishikawa: Kindai University, Nakamachi
Haruhiko Inoue: National Agriculture and Food Research Organization (NARO)
Shusuke Shigeta: Kindai University, Nakamachi
Kento Inoue: Kindai University, Nakamachi
Keita Shimada: Kindai University, Nakamachi
Satomi Yoshimura: Kindai University, Nakamachi
Takumi Takeda: Iwate Biotechnology Research Center
Eiki Yamashita: Osaka University
Toshimichi Fujiwara: Osaka University
Atsushi Nakagawa: Osaka University
Chojiro Kojima: Osaka University
Tsutomu Kawasaki: Kindai University, Nakamachi

Nature Communications, 2022, vol. 13, issue 1, 1-16

Abstract: Abstract The U-box type ubiquitin ligase PUB44 positively regulates pattern-triggered immunity in rice. Here, we identify PBI1, a protein that interacts with PUB44. Crystal structure analysis indicates that PBI1 forms a four-helix bundle structure. PBI1 also interacts with WRKY45, a master transcriptional activator of rice immunity, and negatively regulates its activity. PBI1 is degraded upon perception of chitin, and this is suppressed by silencing of PUB44 or expression of XopP, indicating that PBI1 degradation depends on PUB44. These data suggest that PBI1 suppresses WRKY45 activity when cells are in an unelicited state, and during chitin signaling, PUB44-mediated degradation of PBI1 leads to activation of WRKY45. In addition, chitin-induced MAP kinase activation is required for WRKY45 activation and PBI1 degradation. These results demonstrate that chitin-induced activation of WRKY45 is regulated by the cooperation between MAP kinase-mediated phosphorylation and PUB44-mediated PBI1 degradation.

Date: 2022
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DOI: 10.1038/s41467-022-30131-y

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