Agrobacterium expressing a type III secretion system delivers Pseudomonas effectors into plant cells to enhance transformation

Vidhyavathi Raman, Clemencia M. Rojas, Balaji Vasudevan, Kevin Dunning, Jaydeep Kolape, Sunhee Oh, Jianfei Yun, Lishan Yang, Guangming Li, Bikram D. Pant, Qingzhen Jiang and Kirankumar S. Mysore ()
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Vidhyavathi Raman: Noble Research Institute, LLC
Clemencia M. Rojas: Noble Research Institute, LLC
Balaji Vasudevan: Noble Research Institute, LLC
Kevin Dunning: Noble Research Institute, LLC
Jaydeep Kolape: Noble Research Institute, LLC
Sunhee Oh: Noble Research Institute, LLC
Jianfei Yun: Noble Research Institute, LLC
Lishan Yang: Noble Research Institute, LLC
Guangming Li: Noble Research Institute, LLC
Bikram D. Pant: Noble Research Institute, LLC
Qingzhen Jiang: Noble Research Institute, LLC
Kirankumar S. Mysore: Noble Research Institute, LLC

Nature Communications, 2022, vol. 13, issue 1, 1-14

Abstract: Abstract Agrobacterium-mediated plant transformation (AMT) is the basis of modern-day plant biotechnology. One major drawback of this technology is the recalcitrance of many plant species/varieties to Agrobacterium infection, most likely caused by elicitation of plant defense responses. Here, we develop a strategy to increase AMT by engineering Agrobacterium tumefaciens to express a type III secretion system (T3SS) from Pseudomonas syringae and individually deliver the P. syringae effectors AvrPto, AvrPtoB, or HopAO1 to suppress host defense responses. Using the engineered Agrobacterium, we demonstrate increase in AMT of wheat, alfalfa and switchgrass by ~250%–400%. We also show that engineered A. tumefaciens expressing a T3SS can deliver a plant protein, histone H2A-1, to enhance AMT. This strategy is of great significance to both basic research and agricultural biotechnology for transient and stable transformation of recalcitrant plant species/varieties and to deliver proteins into plant cells in a non-transgenic manner.

Date: 2022
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DOI: 10.1038/s41467-022-30180-3

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