Mechanical loading of intraluminal pressure mediates wound angiogenesis by regulating the TOCA family of F-BAR proteins

Shinya Yuge, Koichi Nishiyama (), Yuichiro Arima, Yasuyuki Hanada, Eri Oguri-Nakamura, Sanshiro Hanada, Tomohiro Ishii, Yuki Wakayama, Urara Hasegawa, Kazuya Tsujita, Ryuji Yokokawa, Takashi Miura, Toshiki Itoh, Kenichi Tsujita, Naoki Mochizuki and Shigetomo Fukuhara ()
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Shinya Yuge: Nippon Medical School
Koichi Nishiyama: Kumamoto University
Yuichiro Arima: Kumamoto University
Yasuyuki Hanada: Kumamoto University
Eri Oguri-Nakamura: Nippon Medical School
Sanshiro Hanada: Kumamoto University
Tomohiro Ishii: Nippon Medical School
Yuki Wakayama: National Cerebral and Cardiovascular Center Research Institute
Urara Hasegawa: Pennsylvania State University
Kazuya Tsujita: Kobe University
Ryuji Yokokawa: Kyoto University
Takashi Miura: Kyushu University
Toshiki Itoh: Kobe University
Kenichi Tsujita: Kumamoto University
Naoki Mochizuki: National Cerebral and Cardiovascular Center Research Institute
Shigetomo Fukuhara: Nippon Medical School

Nature Communications, 2022, vol. 13, issue 1, 1-25

Abstract: Abstract Angiogenesis is regulated in coordinated fashion by chemical and mechanical cues acting on endothelial cells (ECs). However, the mechanobiological mechanisms of angiogenesis remain unknown. Herein, we demonstrate a crucial role of blood flow-driven intraluminal pressure (IP) in regulating wound angiogenesis. During wound angiogenesis, blood flow-driven IP loading inhibits elongation of injured blood vessels located at sites upstream from blood flow, while downstream injured vessels actively elongate. In downstream injured vessels, F-BAR proteins, TOCA1 and CIP4, localize at leading edge of ECs to promote N-WASP-dependent Arp2/3 complex-mediated actin polymerization and front-rear polarization for vessel elongation. In contrast, IP loading expands upstream injured vessels and stretches ECs, preventing leading edge localization of TOCA1 and CIP4 to inhibit directed EC migration and vessel elongation. These data indicate that the TOCA family of F-BAR proteins are key actin regulatory proteins required for directed EC migration and sense mechanical cell stretching to regulate wound angiogenesis.

Date: 2022
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DOI: 10.1038/s41467-022-30197-8

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