Functional and multiscale 3D structural investigation of brain tissue through correlative in vivo physiology, synchrotron microtomography and volume electron microscopy

Carles Bosch (), Tobias Ackels, Alexandra Pacureanu, Yuxin Zhang, Christopher J. Peddie, Manuel Berning, Norman Rzepka, Marie-Christine Zdora, Isabell Whiteley, Malte Storm, Anne Bonnin, Christoph Rau, Troy Margrie, Lucy Collinson and Andreas T. Schaefer ()
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Carles Bosch: The Francis Crick Institute
Tobias Ackels: The Francis Crick Institute
Alexandra Pacureanu: The Francis Crick Institute
Yuxin Zhang: The Francis Crick Institute
Christopher J. Peddie: The Francis Crick Institute
Manuel Berning: Max Planck Institute for Brain Research
Norman Rzepka: Scalable minds GmbH
Marie-Christine Zdora: University College London
Isabell Whiteley: The Francis Crick Institute
Malte Storm: Diamond Light Source, Harwell Science and Innovation Campus
Anne Bonnin: Paul Scherrer Institut
Christoph Rau: Diamond Light Source, Harwell Science and Innovation Campus
Troy Margrie: University College London
Lucy Collinson: The Francis Crick Institute
Andreas T. Schaefer: The Francis Crick Institute

Nature Communications, 2022, vol. 13, issue 1, 1-16

Abstract: Abstract Understanding the function of biological tissues requires a coordinated study of physiology and structure, exploring volumes that contain complete functional units at a detail that resolves the relevant features. Here, we introduce an approach to address this challenge: Mouse brain tissue sections containing a region where function was recorded using in vivo 2-photon calcium imaging were stained, dehydrated, resin-embedded and imaged with synchrotron X-ray computed tomography with propagation-based phase contrast (SXRT). SXRT provided context at subcellular detail, and could be followed by targeted acquisition of multiple volumes using serial block-face electron microscopy (SBEM). In the olfactory bulb, combining SXRT and SBEM enabled disambiguation of in vivo-assigned regions of interest. In the hippocampus, we found that superficial pyramidal neurons in CA1a displayed a larger density of spine apparati than deeper ones. Altogether, this approach can enable a functional and structural investigation of subcellular features in the context of cells and tissues.

Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30199-6

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DOI: 10.1038/s41467-022-30199-6

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