Unlocking the functional potential of polyploid yeasts

Simone Mozzachiodi (), Kristoffer Krogerus, Brian Gibson, Alain Nicolas and Gianni Liti ()
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Simone Mozzachiodi: Université Côte d’Azur, CNRS, INSERM, IRCAN
Kristoffer Krogerus: VTT Technical Research Centre of Finland Ltd
Brian Gibson: VTT Technical Research Centre of Finland Ltd
Alain Nicolas: Université Côte d’Azur, CNRS, INSERM, IRCAN
Gianni Liti: Université Côte d’Azur, CNRS, INSERM, IRCAN

Nature Communications, 2022, vol. 13, issue 1, 1-13

Abstract: Abstract Breeding and domestication have generated widely exploited crops, animals and microbes. However, many Saccharomyces cerevisiae industrial strains have complex polyploid genomes and are sterile, preventing genetic improvement strategies based on breeding. Here, we present a strain improvement approach based on the budding yeasts’ property to promote genetic recombination when meiosis is interrupted and cells return-to-mitotic-growth (RTG). We demonstrate that two unrelated sterile industrial strains with complex triploid and tetraploid genomes are RTG-competent and develop a visual screening for easy and high-throughput identification of recombined RTG clones based on colony phenotypes. Sequencing of the evolved clones reveal unprecedented levels of RTG-induced genome-wide recombination. We generate and extensively phenotype a RTG library and identify clones with superior biotechnological traits. Thus, we propose the RTG-framework as a fully non-GMO workflow to rapidly improve industrial yeasts that can be easily brought to the market.

Date: 2022
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DOI: 10.1038/s41467-022-30221-x

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