Signal transduction in light-oxygen-voltage receptors lacking the active-site glutamine

Julia Dietler, Renate Gelfert, Jennifer Kaiser, Veniamin Borin, Christian Renzl, Sebastian Pilsl, Américo Tavares Ranzani, Andrés García de Fuentes, Tobias Gleichmann, Ralph P. Diensthuber, Michael Weyand, Günter Mayer, Igor Schapiro and Andreas Möglich ()
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Julia Dietler: University of Bayreuth
Renate Gelfert: University of Bayreuth
Jennifer Kaiser: University of Bayreuth
Veniamin Borin: The Hebrew University of Jerusalem
Christian Renzl: University of Bonn
Sebastian Pilsl: University of Bonn
Américo Tavares Ranzani: University of Bayreuth
Andrés García de Fuentes: University of Bayreuth
Tobias Gleichmann: Humboldt-University Berlin
Ralph P. Diensthuber: Humboldt-University Berlin
Michael Weyand: University of Bayreuth
Günter Mayer: University of Bonn
Igor Schapiro: The Hebrew University of Jerusalem
Andreas Möglich: University of Bayreuth

Nature Communications, 2022, vol. 13, issue 1, 1-16

Abstract: Abstract In nature as in biotechnology, light-oxygen-voltage photoreceptors perceive blue light to elicit spatiotemporally defined cellular responses. Photon absorption drives thioadduct formation between a conserved cysteine and the flavin chromophore. An equally conserved, proximal glutamine processes the resultant flavin protonation into downstream hydrogen-bond rearrangements. Here, we report that this glutamine, long deemed essential, is generally dispensable. In its absence, several light-oxygen-voltage receptors invariably retained productive, if often attenuated, signaling responses. Structures of a light-oxygen-voltage paradigm at around 1 Å resolution revealed highly similar light-induced conformational changes, irrespective of whether the glutamine is present. Naturally occurring, glutamine-deficient light-oxygen-voltage receptors likely serve as bona fide photoreceptors, as we showcase for a diguanylate cyclase. We propose that without the glutamine, water molecules transiently approach the chromophore and thus propagate flavin protonation downstream. Signaling without glutamine appears intrinsic to light-oxygen-voltage receptors, which pertains to biotechnological applications and suggests evolutionary descendance from redox-active flavoproteins.

Date: 2022
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DOI: 10.1038/s41467-022-30252-4

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