Biogenesis of a bacterial metabolosome for propanediol utilization

Mengru Yang, Nicolas Wenner, Gregory F. Dykes, Yan Li, Xiaojun Zhu, Yaqi Sun, Fang Huang, Jay C. D. Hinton and Lu-Ning Liu ()
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Mengru Yang: University of Liverpool
Nicolas Wenner: University of Liverpool
Gregory F. Dykes: University of Liverpool
Yan Li: University of Liverpool
Xiaojun Zhu: University of Liverpool
Yaqi Sun: University of Liverpool
Fang Huang: University of Liverpool
Jay C. D. Hinton: University of Liverpool
Lu-Ning Liu: University of Liverpool

Nature Communications, 2022, vol. 13, issue 1, 1-16

Abstract: Abstract Bacterial metabolosomes are a family of protein organelles in bacteria. Elucidating how thousands of proteins self-assemble to form functional metabolosomes is essential for understanding their significance in cellular metabolism and pathogenesis. Here we investigate the de novo biogenesis of propanediol-utilization (Pdu) metabolosomes and characterize the roles of the key constituents in generation and intracellular positioning of functional metabolosomes. Our results demonstrate that the Pdu metabolosome undertakes both “Shell first” and “Cargo first” assembly pathways, unlike the β-carboxysome structural analog which only involves the “Cargo first” strategy. Shell and cargo assemblies occur independently at the cell poles. The internal cargo core is formed through the ordered assembly of multiple enzyme complexes, and exhibits liquid-like properties within the metabolosome architecture. Our findings provide mechanistic insight into the molecular principles driving bacterial metabolosome assembly and expand our understanding of liquid-like organelle biogenesis.

Date: 2022
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DOI: 10.1038/s41467-022-30608-w

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