The 3′ Pol II pausing at replication-dependent histone genes is regulated by Mediator through Cajal bodies’ association with histone locus bodies

Hidefumi Suzuki, Ryota Abe, Miho Shimada, Tomonori Hirose, Hiroko Hirose, Keisuke Noguchi, Yoko Ike, Nanami Yasui, Kazuki Furugori, Yuki Yamaguchi, Atsushi Toyoda, Yutaka Suzuki, Tatsuro Yamamoto, Noriko Saitoh, Shigeo Sato, Chieri Tomomori-Sato, Ronald C. Conaway, Joan W. Conaway and Hidehisa Takahashi ()
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Hidefumi Suzuki: Yokohama City University Graduate School of Medical Science
Ryota Abe: Yokohama City University Graduate School of Medical Science
Miho Shimada: Yokohama City University Graduate School of Medical Science
Tomonori Hirose: Yokohama City University Graduate School of Medical Science
Hiroko Hirose: Yokohama City University Graduate School of Medical Science
Keisuke Noguchi: Yokohama City University Graduate School of Medical Science
Yoko Ike: Yokohama City University Graduate School of Medical Science
Nanami Yasui: Yokohama City University Graduate School of Medical Science
Kazuki Furugori: Yokohama City University Graduate School of Medical Science
Yuki Yamaguchi: Tokyo Institute of Technology
Atsushi Toyoda: National Institute of Genetics
Yutaka Suzuki: The University of Tokyo
Tatsuro Yamamoto: The Cancer Institute of JFCR
Noriko Saitoh: The Cancer Institute of JFCR
Shigeo Sato: Stowers Institute for Medical Research
Chieri Tomomori-Sato: Stowers Institute for Medical Research
Ronald C. Conaway: Stowers Institute for Medical Research
Joan W. Conaway: Stowers Institute for Medical Research
Hidehisa Takahashi: Yokohama City University Graduate School of Medical Science

Nature Communications, 2022, vol. 13, issue 1, 1-24

Abstract: Abstract Non-polyadenylated mRNAs of replication-dependent histones (RDHs) are synthesized by RNA polymerase II (Pol II) at histone locus bodies (HLBs). HLBs frequently associate with Cajal bodies (CBs), in which 3′-end processing factors for RDH genes are enriched; however, this association’s role in transcription termination of RDH genes remains unclear. Here, we show that Pol II pauses immediately upstream of transcript end sites of RDH genes and Mediator plays a role in this Pol II pausing through CBs’ association with HLBs. Disruption of the Mediator docking site for Little elongation complex (LEC)–Cap binding complex (CBC)–Negative elongation factor (NELF), components of CBs, interferes with CBs’ association with HLBs and 3′ Pol II pausing, resulting in increased aberrant unprocessed RDH gene transcripts. Our findings suggest Mediator’s involvement in CBs’ association with HLBs to facilitate 3′ Pol II pausing and subsequent 3′-end processing of RDH genes by supplying 3′-end processing factors.

Date: 2022
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DOI: 10.1038/s41467-022-30632-w

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