P-selectin mobility undergoes a sol-gel transition as it diffuses from exocytosis sites into the cell membrane

Nicola Hellen, Gregory I. Mashanov, Ianina L. Conte, Sophie Trionnaire, Victor Babich, Laura Knipe, Alamin Mohammed, Kazim Ogmen, Silvia Martin-Almedina, Katalin Török, Matthew J. Hannah, Justin E. Molloy () and Tom Carter ()
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Nicola Hellen: The Francis Crick Institute
Gregory I. Mashanov: The Francis Crick Institute
Ianina L. Conte: St Georges University of London
Sophie Trionnaire: St Georges University of London
Victor Babich: Mercy College of Health Sciences
Laura Knipe: The Francis Crick Institute
Alamin Mohammed: St Georges University of London
Kazim Ogmen: St Georges University of London
Silvia Martin-Almedina: St Georges University of London
Katalin Török: St Georges University of London
Matthew J. Hannah: Public Health England
Justin E. Molloy: The Francis Crick Institute
Tom Carter: St Georges University of London

Nature Communications, 2022, vol. 13, issue 1, 1-11

Abstract: Abstract In response to vascular damage, P-selectin molecules are secreted onto the surface of cells that line our blood vessels. They then serve as mechanical anchors to capture leucocytes from the blood stream. Here, we track individual P-selectin molecules released at the surface of live endothelial cells following stimulated secretion. We find P-selectin initially shows fast, unrestricted diffusion but within a few minutes, movement becomes increasingly restricted and ~50% of the molecules become completely immobile; a process similar to a sol-gel transition. We find removal of the extracellular C-type lectin domain (ΔCTLD) and/or intracellular cytoplasmic tail domain (ΔCT) has additive effects on diffusive motion while disruption of the adapter complex, AP2, or removal of cell-surface heparan sulphate restores mobility of full-length P-selectin close to that of ΔCT and ΔCTLD respectively. We have found P-selectin spreads rapidly from sites of exocytosis and evenly decorates the cell surface, but then becomes less mobile and better-suited to its mechanical anchoring function.

Date: 2022
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DOI: 10.1038/s41467-022-30669-x

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