A synaptomic analysis reveals dopamine hub synapses in the mouse striatum

Vincent Paget-Blanc, Marlene E. Pfeffer, Marie Pronot, Paul Lapios, Maria-Florencia Angelo, Roman Walle, Fabrice P. Cordelières, Florian Levet, Stéphane Claverol, Sabrina Lacomme, Mélina Petrel, Christelle Martin, Vincent Pitard, Véronique De Smedt Peyrusse, Thomas Biederer, David Perrais, Pierre Trifilieff and Etienne Herzog ()
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Vincent Paget-Blanc: Univ. Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297
Marlene E. Pfeffer: Univ. Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297
Marie Pronot: Univ. Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297
Paul Lapios: Univ. Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297
Maria-Florencia Angelo: Univ. Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297
Roman Walle: Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286
Fabrice P. Cordelières: Univ. Bordeaux, CNRS, INSERM, Bordeaux Imaging Center, BIC, UAR 3420, US 4
Florian Levet: Univ. Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297
Stéphane Claverol: Univ. Bordeaux, Plateforme Proteome
Sabrina Lacomme: Univ. Bordeaux, CNRS, INSERM, Bordeaux Imaging Center, BIC, UAR 3420, US 4
Mélina Petrel: Univ. Bordeaux, CNRS, INSERM, Bordeaux Imaging Center, BIC, UAR 3420, US 4
Christelle Martin: Univ. Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297
Vincent Pitard: UB’FACSility CNRS UMS 3427, INSERM US 005, Univ. Bordeaux
Véronique De Smedt Peyrusse: Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286
Thomas Biederer: Yale School of Medicine
David Perrais: Univ. Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297
Pierre Trifilieff: Univ. Bordeaux, INRAE, Bordeaux INP, NutriNeuro, UMR 1286
Etienne Herzog: Univ. Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297

Nature Communications, 2022, vol. 13, issue 1, 1-19

Abstract: Abstract Dopamine transmission is involved in reward processing and motor control, and its impairment plays a central role in numerous neurological disorders. Despite its strong pathophysiological relevance, the molecular and structural organization of the dopaminergic synapse remains to be established. Here, we used targeted labelling and fluorescence activated sorting to purify striatal dopaminergic synaptosomes. We provide the proteome of dopaminergic synapses with 57 proteins specifically enriched. Beyond canonical markers of dopamine neurotransmission such as dopamine biosynthetic enzymes and cognate receptors, we validated 6 proteins not previously described as enriched. Moreover, our data reveal the adhesion of dopaminergic synapses to glutamatergic, GABAergic or cholinergic synapses in structures we named “dopamine hub synapses”. At glutamatergic synapses, pre- and postsynaptic markers are significantly increased upon association with dopamine synapses. Dopamine hub synapses may thus support local dopaminergic signalling, complementing volume transmission thought to be the major mechanism by which monoamines modulate network activity.

Date: 2022
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DOI: 10.1038/s41467-022-30776-9

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