Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii

Guifang Lin, Hui Chen, Bin Tian, Sunish K. Sehgal, Lovepreet Singh, Jingzhong Xie, Nidhi Rawat, Philomin Juliana, Narinder Singh, Sandesh Shrestha, Duane L. Wilson, Hannah Shult, Hyeonju Lee, Adam William Schoen, Vijay K. Tiwari, Ravi P. Singh, Mary J. Guttieri, Harold N. Trick, Jesse Poland, Robert L. Bowden, Guihua Bai, Bikram Gill () and Sanzhen Liu ()
Additional contact information
Guifang Lin: Kansas State University
Hui Chen: Kansas State University
Bin Tian: Kansas State University
Sunish K. Sehgal: South Dakota State University
Lovepreet Singh: University of Maryland
Jingzhong Xie: Kansas State University
Nidhi Rawat: University of Maryland
Philomin Juliana: International Maize and Wheat Improvement Center (CIMMYT)
Narinder Singh: Kansas State University
Sandesh Shrestha: Kansas State University
Duane L. Wilson: Kansas State University
Hannah Shult: Kansas State University
Hyeonju Lee: Kansas State University
Adam William Schoen: University of Maryland
Vijay K. Tiwari: University of Maryland
Ravi P. Singh: International Maize and Wheat Improvement Center (CIMMYT)
Mary J. Guttieri: Hard Winter Wheat Genetics Research Unit, USDA-ARS
Harold N. Trick: Kansas State University
Jesse Poland: Kansas State University
Robert L. Bowden: Hard Winter Wheat Genetics Research Unit, USDA-ARS
Guihua Bai: Kansas State University
Bikram Gill: Kansas State University
Sanzhen Liu: Kansas State University

Nature Communications, 2022, vol. 13, issue 1, 1-12

Abstract: Abstract The wheat wild relative Aegilops tauschii was previously used to transfer the Lr42 leaf rust resistance gene into bread wheat. Lr42 confers resistance at both seedling and adult stages, and it is broadly effective against all leaf rust races tested to date. Lr42 has been used extensively in the CIMMYT international wheat breeding program with resulting cultivars deployed in several countries. Here, using a bulked segregant RNA-Seq (BSR-Seq) mapping strategy, we identify three candidate genes for Lr42. Overexpression of a nucleotide-binding site leucine-rich repeat (NLR) gene AET1Gv20040300 induces strong resistance to leaf rust in wheat and a mutation of the gene disrupted the resistance. The Lr42 resistance allele is rare in Ae. tauschii and likely arose from ectopic recombination. Cloning of Lr42 provides diagnostic markers and over 1000 CIMMYT wheat lines carrying Lr42 have been developed documenting its widespread use and impact in crop improvement.

Date: 2022
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DOI: 10.1038/s41467-022-30784-9

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