A multifunctional system for genome editing and large-scale interspecies gene transfer

Marc Teufel, Carlo A. Klein, Maurice Mager and Patrick Sobetzko ()
Additional contact information
Marc Teufel: Philipps Universität Marburg, Synthetic Microbiology Center Marburg (SYNMIKRO)
Carlo A. Klein: Philipps Universität Marburg, Synthetic Microbiology Center Marburg (SYNMIKRO)
Maurice Mager: Philipps Universität Marburg, Synthetic Microbiology Center Marburg (SYNMIKRO)
Patrick Sobetzko: Philipps Universität Marburg, Synthetic Microbiology Center Marburg (SYNMIKRO)

Nature Communications, 2022, vol. 13, issue 1, 1-19

Abstract: Abstract CRISPR SWAPnDROP extends the limits of genome editing to large-scale in-vivo DNA transfer between bacterial species. Its modular platform approach facilitates species specific adaptation to confer genome editing in various species. In this study, we show the implementation of the CRISPR SWAPnDROP concept for the model organism Escherichia coli, the fast growing Vibrio natriegens and the plant pathogen Dickeya dadantii. We demonstrate the excision, transfer and integration of large chromosomal regions between E. coli, V. natriegens and D. dadantii without size-limiting intermediate DNA extraction. CRISPR SWAPnDROP also provides common genome editing approaches comprising scarless, marker-free, iterative and parallel insertions and deletions. The modular character facilitates DNA library applications, and recycling of standardized parts. Its multi-color scarless co-selection system significantly improves editing efficiency and provides visual quality controls throughout the assembly and editing process.

Date: 2022
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-022-30843-1 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30843-1

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-022-30843-1

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().