Salivary gland organoid culture maintains distinct glandular properties of murine and human major salivary glands

Yeo-Jun Yoon, Donghyun Kim, Kwon Yong Tak, Seungyeon Hwang, Jisun Kim, Nam Suk Sim, Jae-Min Cho, Dojin Choi, Youngmi Ji, Junho K. Hur, Hyunki Kim, Jong-Eun Park and Jae-Yol Lim ()
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Yeo-Jun Yoon: Yonsei University College of Medicine
Donghyun Kim: Yonsei University College of Medicine
Kwon Yong Tak: Korean Advanced Institute of Science and Technology
Seungyeon Hwang: Yonsei University College of Medicine
Jisun Kim: Yonsei University College of Medicine
Nam Suk Sim: Yonsei University College of Medicine
Jae-Min Cho: Yonsei University College of Medicine
Dojin Choi: Yonsei University College of Medicine
Youngmi Ji: National Institute of Dental and Craniofacial Research, NIH
Junho K. Hur: Hanyang University
Hyunki Kim: Yonsei University College of Medicine
Jong-Eun Park: Korean Advanced Institute of Science and Technology
Jae-Yol Lim: Yonsei University College of Medicine

Nature Communications, 2022, vol. 13, issue 1, 1-16

Abstract: Abstract Salivary glands that produce and secrete saliva, which is essential for lubrication, digestion, immunity, and oral homeostasis, consist of diverse cells. The long-term maintenance of diverse salivary gland cells in organoids remains problematic. Here, we establish long-term murine and human salivary gland organoid cultures. Murine and human salivary gland organoids express gland-specific genes and proteins of acinar, myoepithelial, and duct cells, and exhibit gland functions when stimulated with neurotransmitters. Furthermore, human salivary gland organoids are established from isolated basal or luminal cells, retaining their characteristics. Single-cell RNA sequencing also indicates that human salivary gland organoids contain heterogeneous cell types and replicate glandular diversity. Our protocol also enables the generation of tumoroid cultures from benign and malignant salivary gland tumor types, in which tumor-specific gene signatures are well-conserved. In this study, we provide an experimental platform for the exploration of precision medicine in the era of tissue regeneration and anticancer treatment.

Date: 2022
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DOI: 10.1038/s41467-022-30934-z

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