Taf2 mediates DNA binding of Taf14

Brianna J. Klein, Jordan T. Feigerle, Jibo Zhang, Christopher C. Ebmeier, Lixin Fan, Rohit K. Singh, Wesley W. Wang, Lauren R. Schmitt, Thomas Lee, Kirk C. Hansen, Wenshe R. Liu, Yun-Xing Wang, Brian D. Strahl, P. Anthony Weil and Tatiana G. Kutateladze ()
Additional contact information
Brianna J. Klein: University of Colorado School of Medicine
Jordan T. Feigerle: Stanford University
Jibo Zhang: The University of North Carolina School of Medicine
Christopher C. Ebmeier: University of Colorado
Lixin Fan: SAXS Core Facility of the National Cancer Institute
Rohit K. Singh: University of Colorado School of Medicine
Wesley W. Wang: Texas A&M University
Lauren R. Schmitt: University of Colorado School of Medicine
Thomas Lee: SAXS Core Facility of the National Cancer Institute
Kirk C. Hansen: University of Colorado School of Medicine
Wenshe R. Liu: Texas A&M University
Yun-Xing Wang: National Institutes of Health
Brian D. Strahl: The University of North Carolina School of Medicine
P. Anthony Weil: Vanderbilt University School of Medicine
Tatiana G. Kutateladze: University of Colorado School of Medicine

Nature Communications, 2022, vol. 13, issue 1, 1-11

Abstract: Abstract The assembly and function of the yeast general transcription factor TFIID complex requires specific contacts between its Taf14 and Taf2 subunits, however, the mechanism underlying these contacts remains unclear. Here, we determined the molecular and structural basis by which the YEATS and ET domains of Taf14 bind to the C-terminal tail of Taf2 and identified a unique DNA-binding activity of the linker region connecting the two domains. We show that in the absence of ligands the linker region of Taf14 is occluded by the surrounding domains, and therefore the DNA binding function of Taf14 is autoinhibited. Binding of Taf2 promotes a conformational rearrangement in Taf14, resulting in a release of the linker for the engagement with DNA and the nucleosome. Genetic in vivo data indicate that the association of Taf14 with both Taf2 and DNA is essential for transcriptional regulation. Our findings provide a basis for deciphering the role of individual TFIID subunits in mediating gene transcription.

Date: 2022
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DOI: 10.1038/s41467-022-30937-w

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