Healthy cloned offspring derived from freeze-dried somatic cells

Sayaka Wakayama (), Daiyu Ito, Erika Hayashi, Takashi Ishiuchi and Teruhiko Wakayama ()
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Sayaka Wakayama: University of Yamanashi
Daiyu Ito: University of Yamanashi
Erika Hayashi: University of Yamanashi
Takashi Ishiuchi: University of Yamanashi
Teruhiko Wakayama: University of Yamanashi

Nature Communications, 2022, vol. 13, issue 1, 1-9

Abstract: Abstract Maintaining biodiversity is an essential task, but storing germ cells as genetic resources using liquid nitrogen is difficult, expensive, and easily disrupted during disasters. Our aim is to generate cloned mice from freeze-dried somatic cell nuclei, preserved at −30 °C for up to 9 months after freeze drying treatment. All somatic cells died after freeze drying, and nucleic DNA damage significantly increased. However, after nuclear transfer, we produced cloned blastocysts from freeze-dried somatic cells, and established nuclear transfer embryonic stem cell lines. Using these cells as nuclear donors for re-cloning, we obtained healthy cloned female and male mice with a success rate of 0.2–5.4%. Here, we show that freeze-dried somatic cells can produce healthy, fertile clones, suggesting that this technique may be important for the establishment of alternative, cheaper, and safer liquid nitrogen-free bio-banking solutions.

Date: 2022
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DOI: 10.1038/s41467-022-31216-4

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