Mutations in DNA polymerase δ subunit 1 co-segregate with CMD2-type resistance to Cassava Mosaic Geminiviruses

Yi-Wen Lim, Ben N. Mansfeld, Pascal Schläpfer, Kerrigan B. Gilbert, Narayanan N. Narayanan, Weihong Qi, Qi Wang, Zhenhui Zhong, Adam Boyher, Jackson Gehan, Getu Beyene, Zuh-Jyh Daniel Lin, Williams Esuma, Suhua Feng, Christelle Chanez, Nadine Eggenberger, Gerald Adiga, Titus Alicai, Steven E. Jacobsen, Nigel J. Taylor, Wilhelm Gruissem () and Rebecca S. Bart ()
Additional contact information
Yi-Wen Lim: ETH Zürich
Ben N. Mansfeld: Donald Danforth Plant Science Center
Pascal Schläpfer: ETH Zürich
Kerrigan B. Gilbert: Donald Danforth Plant Science Center
Narayanan N. Narayanan: Donald Danforth Plant Science Center
Weihong Qi: ETH Zurich and University of Zurich
Qi Wang: Donald Danforth Plant Science Center
Zhenhui Zhong: University of California Los Angeles
Adam Boyher: Donald Danforth Plant Science Center
Jackson Gehan: Donald Danforth Plant Science Center
Getu Beyene: Donald Danforth Plant Science Center
Zuh-Jyh Daniel Lin: Donald Danforth Plant Science Center
Williams Esuma: National Crops Resources Research Institute
Suhua Feng: University of California Los Angeles
Christelle Chanez: ETH Zürich
Nadine Eggenberger: ETH Zürich
Gerald Adiga: National Crops Resources Research Institute
Titus Alicai: National Crops Resources Research Institute
Steven E. Jacobsen: University of California Los Angeles
Nigel J. Taylor: Donald Danforth Plant Science Center
Wilhelm Gruissem: ETH Zürich
Rebecca S. Bart: Donald Danforth Plant Science Center

Nature Communications, 2022, vol. 13, issue 1, 1-11

Abstract: Abstract Cassava mosaic disease (CMD) suppresses cassava yields across the tropics. The dominant CMD2 locus confers resistance to cassava mosaic geminiviruses. It has been reported that CMD2-type landraces lose resistance after regeneration through de novo morphogenesis. As full genome bisulfite sequencing failed to uncover an epigenetic mechanism for this loss of resistance, whole genome sequencing and genetic variant analysis was performed and the CMD2 locus was fine-mapped to a 190 kilobase interval. Collectively, these data indicate that CMD2-type resistance is caused by a nonsynonymous, single nucleotide polymorphism in DNA polymerase δ subunit 1 (MePOLD1) located within this region. Virus-induced gene silencing of MePOLD1 in a CMD-susceptible cassava variety produced a recovery phenotype typical of CMD2-type resistance. Analysis of other CMD2-type cassava varieties identified additional candidate resistance alleles within MePOLD1. Genetic variation of MePOLD1, therefore, could represent an important genetic resource for resistance breeding and/or genome editing, and elucidating mechanisms of resistance to geminiviruses.

Date: 2022
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DOI: 10.1038/s41467-022-31414-0

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