Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR

Kenneth Danh, Donna Grace Karp, Malvika Singhal, Akshaya Tankasala, David Gebhart, Felipe Jesus Cortez, Devangkumar Tandel, Peter V. Robinson, David Seftel, Mars Stone, Graham Simmons, Anil Bagri, Martin A. Schreiber, Andreas Buser, Andreas Holbro, Manuel Battegay, Mary Kate Morris, Carl Hanson, John R. Mills, Dane Granger, Elitza S. Theel, James R. Stubbs, Laurence M. Corash and Cheng-ting Tsai ()
Additional contact information
Kenneth Danh: Enable Biosciences Inc
Donna Grace Karp: Enable Biosciences Inc
Malvika Singhal: Enable Biosciences Inc
Akshaya Tankasala: Enable Biosciences Inc
David Gebhart: Enable Biosciences Inc
Felipe Jesus Cortez: Enable Biosciences Inc
Devangkumar Tandel: Enable Biosciences Inc
Peter V. Robinson: Enable Biosciences Inc
David Seftel: Enable Biosciences Inc
Mars Stone: Vitalant Research Institute
Graham Simmons: Vitalant Research Institute
Anil Bagri: Cerus Corporation
Martin A. Schreiber: Oregon Health & Science University
Andreas Buser: University Hospital Basel, University of Basel
Andreas Holbro: University Hospital Basel, University of Basel
Manuel Battegay: University Hospital Basel, University of Basel
Mary Kate Morris: California Department of Public Health
Carl Hanson: California Department of Public Health
John R. Mills: Mayo Clinic
Dane Granger: Mayo Clinic
Elitza S. Theel: Mayo Clinic
James R. Stubbs: Mayo Clinic
Laurence M. Corash: Cerus Corporation
Cheng-ting Tsai: Enable Biosciences Inc

Nature Communications, 2022, vol. 13, issue 1, 1-8

Abstract: Abstract An easily implementable serological assay to accurately detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) neutralizing antibodies is urgently needed to better track herd immunity, vaccine efficacy and vaccination rates. Herein, we report the Split-Oligonucleotide Neighboring Inhibition Assay (SONIA) which uses real-time qPCR to measure the ability of neutralizing antibodies to block binding between DNA-barcoded viral spike protein subunit 1 and the human angiotensin-converting enzyme 2 receptor protein. The SONIA neutralizing antibody assay using finger-prick dried blood spots displays 91–97% sensitivity and 100% specificity in comparison to the live-virus neutralization assays using matched serum specimens for multiple SARS-CoV-2 variants-of-concern. The multiplex version of this neutralizing antibody assay, using easily collectable finger-prick dried blood spots, can be a valuable tool to help reveal the impact of age, pre-existing health conditions, waning immunity, different vaccination schemes and the emergence of new variants-of-concern.

Date: 2022
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DOI: 10.1038/s41467-022-31796-1

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