Auxotrophic and prototrophic conditional genetic networks reveal the rewiring of transcription factors in Escherichia coli

Alla Gagarinova, Ali Hosseinnia, Matineh Rahmatbakhsh, Zoe Istace, Sadhna Phanse, Mohamed Taha Moutaoufik, Mara Zilocchi, Qingzhou Zhang, Hiroyuki Aoki, Matthew Jessulat, Sunyoung Kim, Khaled A. Aly and Mohan Babu ()
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Alla Gagarinova: University of Regina
Ali Hosseinnia: University of Regina
Matineh Rahmatbakhsh: University of Regina
Zoe Istace: University of Regina
Sadhna Phanse: University of Regina
Mohamed Taha Moutaoufik: University of Regina
Mara Zilocchi: University of Regina
Qingzhou Zhang: University of Regina
Hiroyuki Aoki: University of Regina
Matthew Jessulat: University of Regina
Sunyoung Kim: University of Regina
Khaled A. Aly: University of Regina
Mohan Babu: University of Regina

Nature Communications, 2022, vol. 13, issue 1, 1-16

Abstract: Abstract Bacterial transcription factors (TFs) are widely studied in Escherichia coli. Yet it remains unclear how individual genes in the underlying pathways of TF machinery operate together during environmental challenge. Here, we address this by applying an unbiased, quantitative synthetic genetic interaction (GI) approach to measure pairwise GIs among all TF genes in E. coli under auxotrophic (rich medium) and prototrophic (minimal medium) static growth conditions. The resulting static and differential GI networks reveal condition-dependent GIs, widespread changes among TF genes in metabolism, and new roles for uncharacterized TFs (yjdC, yneJ, ydiP) as regulators of cell division, putrescine utilization pathway, and cold shock adaptation. Pan-bacterial conservation suggests TF genes with GIs are co-conserved in evolution. Together, our results illuminate the global organization of E. coli TFs, and remodeling of genetic backup systems for TFs under environmental change, which is essential for controlling the bacterial transcriptional regulatory circuits.

Date: 2022
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DOI: 10.1038/s41467-022-31819-x

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