Rep15 interacts with several Rab GTPases and has a distinct fold for a Rab effector

Amrita Rai (), Anurag K. Singh, Nathalie Bleimling, Guido Posern, Ingrid R. Vetter and Roger S. Goody ()
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Amrita Rai: Max Planck Institute of Molecular Physiology
Anurag K. Singh: Martin Luther University Halle-Wittenberg
Nathalie Bleimling: Max Planck Institute of Molecular Physiology
Guido Posern: Martin Luther University Halle-Wittenberg
Ingrid R. Vetter: Max Planck Institute of Molecular Physiology
Roger S. Goody: Max Planck Institute of Molecular Physiology

Nature Communications, 2022, vol. 13, issue 1, 1-17

Abstract: Abstract In their GTP-bound (active) form, Rab proteins interact with effector proteins that control downstream signaling. One such Rab15 effector is Rep15, which is known to have a role in receptor recycling from the endocytic recycling compartment but otherwise remains poorly characterized. Here, we report the characterization of the Rep15:Rab15 interaction and identification of Rab3 paralogs and Rab34 as Rep15 interacting partners from a yeast two-hybrid assay. Biochemical validation of the interactions is presented and crystal structures of the Rep15:Rab3B and Rep15:Rab3C complexes provide additional mechanistic insight. We find that Rep15 adopts a globular structure that is distinct from other reported Rab15, Rab3 and Rab34 effectors. Structure-based mutagenesis experiments explain the Rep15:Rab interaction specificity. Rep15 depletion in U138MG glioblastoma cells impairs cell proliferation, cell migration and receptor recycling, underscoring the need for further clarification of the role of Rep15 in cancer.

Date: 2022
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DOI: 10.1038/s41467-022-31831-1

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