A time-resolved multi-omics atlas of Acanthamoeba castellanii encystment

Clément Bernard, Marie Locard-Paulet, Cyril Noël, Magalie Duchateau, Quentin Giai Gianetto, Bouziane Moumen, Thomas Rattei, Yann Hechard, Lars Juhl Jensen, Mariette Matondo and Ascel Samba-Louaka ()
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Clément Bernard: Université de Poitiers, UMR CNRS
Marie Locard-Paulet: University of Copenhagen
Cyril Noël: Centre Bretagne
Magalie Duchateau: Université de Paris, Proteomics Platform, Mass Spectrometry for Biology Unit, UAR2024
Quentin Giai Gianetto: Université de Paris, Proteomics Platform, Mass Spectrometry for Biology Unit, UAR2024
Bouziane Moumen: Université de Poitiers, UMR CNRS
Thomas Rattei: University of Vienna
Yann Hechard: Université de Poitiers, UMR CNRS
Lars Juhl Jensen: University of Copenhagen
Mariette Matondo: Université de Paris, Proteomics Platform, Mass Spectrometry for Biology Unit, UAR2024
Ascel Samba-Louaka: Université de Poitiers, UMR CNRS

Nature Communications, 2022, vol. 13, issue 1, 1-14

Abstract: Abstract Encystment is a common stress response of most protists, including free-living amoebae. Cyst formation protects the amoebae from eradication and can increase virulence of the bacteria they harbor. Here, we mapped the global molecular changes that occur in the facultatively pathogenic amoeba Acanthamoeba castellanii during the early steps of the poorly understood process of encystment. By performing transcriptomic, proteomic, and phosphoproteomic experiments during encystment, we identified more than 150,000 previously undescribed transcripts and thousands of protein sequences absent from the reference genome. These results provide molecular details to the regulation of expected biological processes, such as cell proliferation shutdown, and reveal new insights such as a rapid phospho-regulation of sites involved in cytoskeleton remodeling and translation regulation. This work constitutes the first time-resolved molecular atlas of an encysting organism and a useful resource for further investigation of amoebae encystment to allow for a better control of pathogenic amoebae.

Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31832-0

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DOI: 10.1038/s41467-022-31832-0

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