Actin nano-architecture of phagocytic podosomes

J. Cody Herron, Shiqiong Hu, Takashi Watanabe, Ana T. Nogueira, Bei Liu, Megan E. Kern, Jesse Aaron, Aaron Taylor, Michael Pablo, Teng-Leong Chew, Timothy C. Elston () and Klaus M. Hahn ()
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J. Cody Herron: University of North Carolina at Chapel Hill
Shiqiong Hu: University of North Carolina at Chapel Hill
Takashi Watanabe: University of North Carolina at Chapel Hill
Ana T. Nogueira: University of North Carolina at Chapel Hill
Bei Liu: University of North Carolina at Chapel Hill
Megan E. Kern: University of North Carolina at Chapel Hill
Jesse Aaron: Howard Hughes Medical Institute Janelia Research Campus
Aaron Taylor: Howard Hughes Medical Institute Janelia Research Campus
Michael Pablo: University of North Carolina at Chapel Hill
Teng-Leong Chew: Howard Hughes Medical Institute Janelia Research Campus
Timothy C. Elston: University of North Carolina at Chapel Hill
Klaus M. Hahn: University of North Carolina at Chapel Hill

Nature Communications, 2022, vol. 13, issue 1, 1-16

Abstract: Abstract Podosomes are actin-enriched adhesion structures important for multiple cellular processes, including migration, bone remodeling, and phagocytosis. Here, we characterize the structure and organization of phagocytic podosomes using interferometric photoactivated localization microscopy, a super-resolution microscopy technique capable of 15–20 nm resolution, together with structured illumination microscopy and localization-based super-resolution microscopy. Phagocytic podosomes are observed during frustrated phagocytosis, a model in which cells attempt to engulf micropatterned IgG antibodies. For circular patterns, this results in regular arrays of podosomes with well-defined geometry. Using persistent homology, we develop a pipeline for semi-automatic identification and measurement of podosome features. These studies reveal an hourglass shape of the podosome actin core, a protruding knob at the bottom of the core, and two actin networks extending from the core. Additionally, the distributions of paxillin, talin, myosin II, α-actinin, cortactin, and microtubules relative to actin are characterized.

Date: 2022
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DOI: 10.1038/s41467-022-32038-0

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