Phosphatidylinositol-4-phosphate controls autophagosome formation in Arabidopsis thaliana

Rodrigo Enrique Gomez, Clément Chambaud, Josselin Lupette, Julie Castets, Stéphanie Pascal, Lysiane Brocard, Lise Noack, Yvon Jaillais, Jérôme Joubès and Amélie Bernard ()
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Rodrigo Enrique Gomez: Univ. Bordeaux
Clément Chambaud: Univ. Bordeaux
Josselin Lupette: Univ. Bordeaux
Julie Castets: Univ. Bordeaux
Stéphanie Pascal: Univ. Bordeaux
Lysiane Brocard: Univ. Bordeaux
Lise Noack: Univ. Lyon, ENS de Lyon, CNRS, INRAE
Yvon Jaillais: Univ. Lyon, ENS de Lyon, CNRS, INRAE
Jérôme Joubès: Univ. Bordeaux
Amélie Bernard: Univ. Bordeaux

Nature Communications, 2022, vol. 13, issue 1, 1-15

Abstract: Abstract Autophagy is an intracellular degradation mechanism critical for plant acclimation to environmental stresses. Central to autophagy is the formation of specialized vesicles, the autophagosomes, which target and deliver cargo to the lytic vacuole. How autophagosomes form in plant cells remains poorly understood. Here, we uncover the importance of the lipid phosphatidylinositol-4-phosphate in autophagy using pharmacological and genetical approaches. Combining biochemical and live-microscopy analyses, we show that PI4K activity is required for early stages of autophagosome formation. Further, our results show that the plasma membrane-localized PI4Kα1 is involved in autophagy and that a substantial portion of autophagy structures are found in proximity to the PI4P-enriched plasma membrane. Together, our study unravels critical insights into the molecular determinants of autophagy, proposing a model whereby the plasma membrane provides PI4P to support the proper assembly and expansion of the phagophore thus governing autophagosome formation in Arabidopsis.

Date: 2022
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DOI: 10.1038/s41467-022-32109-2

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