Three-dimensional electron ptychography of organic–inorganic hybrid nanostructures

Zhiyuan Ding, Si Gao, Weina Fang, Chen Huang, Liqi Zhou, Xudong Pei, Xiaoguo Liu, Xiaoqing Pan, Chunhai Fan, Angus I. Kirkland and Peng Wang ()
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Zhiyuan Ding: Nanjing University
Si Gao: Nanjing University
Weina Fang: East China Normal University
Chen Huang: University of Oxford
Liqi Zhou: Nanjing University
Xudong Pei: Nanjing University
Xiaoguo Liu: Shanghai Jiao Tong University
Xiaoqing Pan: University of California
Chunhai Fan: Shanghai Jiao Tong University
Angus I. Kirkland: University of Oxford
Peng Wang: Nanjing University

Nature Communications, 2022, vol. 13, issue 1, 1-9

Abstract: Abstract Three dimensional scaffolded DNA origami with inorganic nanoparticles has been used to create tailored multidimensional nanostructures. However, the image contrast of DNA is poorer than those of the heavy nanoparticles in conventional transmission electron microscopy at high defocus so that the biological and non-biological components in 3D scaffolds cannot be simultaneously resolved using tomography of samples in a native state. We demonstrate the use of electron ptychography to recover high contrast phase information from all components in a DNA origami scaffold without staining. We further quantitatively evaluate the enhancement of contrast in comparison with conventional transmission electron microscopy. In addition, We show that for ptychography post-reconstruction focusing simplifies the workflow and reduces electron dose and beam damage.

Date: 2022
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DOI: 10.1038/s41467-022-32548-x

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