Comprehensive structural assignment of glycosaminoglycan oligo- and polysaccharides by protein nanopore

Parisa Bayat, Charlotte Rambaud, Bernard Priem, Matthieu Bourderioux, Mélanie Bilong, Salomé Poyer, Manuela Pastoriza-Gallego, Abdelghani Oukhaled, Jérôme Mathé () and Régis Daniel ()
Additional contact information
Parisa Bayat: Université Paris-Saclay, Univ Evry, CNRS, LAMBE
Charlotte Rambaud: Université Paris-Saclay, Univ Evry, CNRS, LAMBE
Bernard Priem: University Grenoble Alpes
Matthieu Bourderioux: Université Paris-Saclay, Univ Evry, CNRS, LAMBE
Mélanie Bilong: Université Paris-Saclay, Univ Evry, CNRS, LAMBE
Salomé Poyer: Université Paris-Saclay, Univ Evry, CNRS, LAMBE
Manuela Pastoriza-Gallego: CY Cergy Paris Université, CNRS, LAMBE
Abdelghani Oukhaled: CY Cergy Paris Université, CNRS, LAMBE
Jérôme Mathé: Université Paris-Saclay, Univ Evry, CNRS, LAMBE
Régis Daniel: Université Paris-Saclay, Univ Evry, CNRS, LAMBE

Nature Communications, 2022, vol. 13, issue 1, 1-12

Abstract: Abstract Glycosaminoglycans are highly anionic functional polysaccharides with information content in their structure that plays a major role in the communication between the cell and the extracellular environment. The study presented here reports the label-free detection and analysis of glycosaminoglycan molecules at the single molecule level using sensing by biological nanopore, thus addressing the need to decipher structural information in oligo- and polysaccharide sequences, which remains a major challenge for glycoscience. We demonstrate that a wild-type aerolysin nanopore can detect and characterize glycosaminoglycan oligosaccharides with various sulfate patterns, osidic bonds and epimers of uronic acid residues. Size discrimination of tetra- to icosasaccharides from heparin, chondroitin sulfate and dermatan sulfate was investigated and we show that different contents and distributions of sulfate groups can be detected. Remarkably, differences in α/β anomerization and 1,4/1,3 osidic linkages can also be detected in heparosan and hyaluronic acid, as well as the subtle difference between the glucuronic/iduronic epimers in chondroitin and dermatan sulfate. Although, at this stage, discrimination of each of the constituent units of GAGs is not yet achieved at the single-molecule level, the resolution reached in this study is an essential step toward this ultimate goal.

Date: 2022
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-022-32800-4 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32800-4

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-022-32800-4

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().